• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Qnr 对质粒拓扑异构酶 Gyrase 毒素 CcdB 和 ParE 的影响。

Effect of Qnr on Plasmid Gyrase Toxins CcdB and ParE.

机构信息

Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA Inje University College of Medicine, Busan, South Korea.

Lahey Hospital and Medical Center, Burlington, Massachusetts, USA

出版信息

Antimicrob Agents Chemother. 2015 Aug;59(8):5078-9. doi: 10.1128/AAC.00524-15. Epub 2015 Jun 8.

DOI:10.1128/AAC.00524-15
PMID:26055367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4505230/
Abstract

Plasmid toxins CcdB and ParE are part of addiction systems promoting plasmid maintenance. Both target host DNA gyrase, as do quinolones and plasmid-determined Qnr proteins that protect gyrase from quinolone inhibition. We cloned qnrB4, qnrS1, ccdB, parE, and the antitoxin-encoding genes ccdA and parD on compatible plasmids and tested them in combination. CcdB and ParE had no specific effect on quinolone susceptibility or Qnr protection, and Qnr did not act as a CcdB or ParE antitoxin.

摘要

质粒毒素 CcdB 和 ParE 是促进质粒维持的成瘾系统的一部分。两者都靶向宿主 DNA 回旋酶，就像喹诺酮类药物和质粒决定的 Qnr 蛋白一样，它们可以保护回旋酶免受喹诺酮类药物的抑制。我们将 qnrB4、qnrS1、ccdB、parE 以及抗毒素编码基因 ccdA 和 parD 克隆到相容质粒上，并对它们进行了组合测试。CcdB 和 ParE 对喹诺酮类药物的敏感性或 Qnr 保护没有特异性作用，而 Qnr 也不作为 CcdB 或 ParE 的抗毒素。

相似文献

1
Effect of Qnr on Plasmid Gyrase Toxins CcdB and ParE.Qnr 对质粒拓扑异构酶 Gyrase 毒素 CcdB 和 ParE 的影响。
Antimicrob Agents Chemother. 2015 Aug;59(8):5078-9. doi: 10.1128/AAC.00524-15. Epub 2015 Jun 8.
2
Diverse phenotypic and genotypic characterization among clinical Klebsiella pneumoniae and Escherichia coli isolates carrying plasmid-mediated quinolone resistance determinants.携带质粒介导喹诺酮类耐药决定因子的临床肺炎克雷伯菌和大肠埃希菌分离株的表型和基因型特征多样化。
Microb Drug Resist. 2011 Sep;17(3):363-7. doi: 10.1089/mdr.2011.0034. Epub 2011 May 12.
3
Prevalence of plasmid-mediated quinolone resistance and mutations in the gyrase and topoisomerase IV genes in Salmonella isolated from 12 tertiary-care hospitals in Korea.韩国 12 家三级保健医院分离的沙门氏菌中质粒介导的喹诺酮耐药性及其在拓扑异构酶和回旋酶基因上的突变流行率。
Microb Drug Resist. 2011 Dec;17(4):551-7. doi: 10.1089/mdr.2011.0095. Epub 2011 Aug 10.
4
Mechanism of plasmid-mediated quinolone resistance.质粒介导的喹诺酮耐药机制。
Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5638-42. doi: 10.1073/pnas.082092899. Epub 2002 Apr 9.
5
Contribution of mutations in DNA gyrase and topoisomerase IV genes to ciprofloxacin resistance in Escherichia coli clinical isolates.DNA 回旋酶和拓扑异构酶 IV 基因突变对大肠杆菌临床分离株中环丙沙星耐药性的贡献。
Int J Antimicrob Agents. 2011 Mar;37(3):253-5. doi: 10.1016/j.ijantimicag.2010.11.022. Epub 2011 Jan 13.
6
Occurrence of fluoroquinolones and fluoroquinolone-resistance genes in the aquatic environment.氟喹诺酮类药物和氟喹诺酮类耐药基因在水环境中的出现。
Sci Total Environ. 2013 Feb 1;444:508-14. doi: 10.1016/j.scitotenv.2012.11.077. Epub 2013 Jan 3.
7
Prevalence of plasmid-mediated quinolone resistance determinants qnr and aac(6')-Ib-cr in Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases in Spain.西班牙产超广谱β-内酰胺酶的大肠埃希菌和肺炎克雷伯菌中质粒介导的喹诺酮耐药决定因子 qnr 和 aac(6')-Ib-cr 的流行情况。
Int J Antimicrob Agents. 2012 May;39(5):431-4. doi: 10.1016/j.ijantimicag.2011.12.009. Epub 2012 Feb 22.
8
Interaction of the plasmid-encoded quinolone resistance protein Qnr with Escherichia coli DNA gyrase.质粒编码的喹诺酮耐药蛋白Qnr与大肠杆菌DNA促旋酶的相互作用。
Antimicrob Agents Chemother. 2005 Jan;49(1):118-25. doi: 10.1128/AAC.49.1.118-125.2005.
9
Emergence of a new mutation and its accumulation in the topoisomerase IV gene confers high levels of resistance to fluoroquinolones in Escherichia coli isolates.新突变的出现及其在拓扑异构酶 IV 基因中的积累赋予了大肠杆菌分离株对氟喹诺酮类药物的高度耐药性。
Int J Antimicrob Agents. 2010 Jan;35(1):76-9. doi: 10.1016/j.ijantimicag.2009.08.003. Epub 2009 Sep 24.
10
Dual targeting of topoisomerase IV and gyrase to reduce mutant selection: direct testing of the paradigm by using WCK-1734, a new fluoroquinolone, and ciprofloxacin.双重靶向拓扑异构酶IV和回旋酶以减少突变选择：使用新型氟喹诺酮类药物WCK-1734和环丙沙星对该范例进行直接测试。
Antimicrob Agents Chemother. 2005 May;49(5):1949-56. doi: 10.1128/AAC.49.5.1949-1956.2005.

引用本文的文献

1
Transcriptional expression of secondary resistance genes ccdB and repA2 is enhanced in presence of cephalosporin and carbapenem in Escherichia coli.在大肠埃希菌中，头孢菌素和碳青霉烯类抗生素的存在会增强次级耐药基因 ccdB 和 repA2 的转录表达。
BMC Microbiol. 2021 Mar 9;21(1):79. doi: 10.1186/s12866-021-02136-y.
2
Pentapeptide repeat protein QnrB1 requires ATP hydrolysis to rejuvenate poisoned gyrase complexes.五肽重复蛋白 QnrB1 需要 ATP 水解来使被毒化的拓扑异构酶复合物恢复活力。
Nucleic Acids Res. 2021 Feb 22;49(3):1581-1596. doi: 10.1093/nar/gkaa1266.
3
Transferable Mechanisms of Quinolone Resistance from 1998 Onward.1998 年以来喹诺酮类耐药的可转移机制。
Clin Microbiol Rev. 2019 Aug 14;32(4). doi: 10.1128/CMR.00007-19. Print 2019 Sep 18.
4
The toxin from a ParDE toxin-antitoxin system found in Pseudomonas aeruginosa offers protection to cells challenged with anti-gyrase antibiotics.铜绿假单胞菌中发现的 ParDE 毒素-抗毒素系统的毒素为对抗螺旋酶抗生素的细胞提供了保护。
Mol Microbiol. 2019 Feb;111(2):441-454. doi: 10.1111/mmi.14165. Epub 2018 Dec 5.
5
Protective effect of Qnr on agents other than quinolones that target DNA gyrase.Qnr对除喹诺酮类药物外其他靶向DNA促旋酶的药物的保护作用。
Antimicrob Agents Chemother. 2015 Nov;59(11):6689-95. doi: 10.1128/AAC.01292-15. Epub 2015 Aug 3.

本文引用的文献

1
Prevalence of plasmid-mediated quinolone resistance determinants over a 9-year period.9年期间质粒介导的喹诺酮耐药决定因素的流行情况。
Antimicrob Agents Chemother. 2009 Feb;53(2):639-45. doi: 10.1128/AAC.01051-08. Epub 2008 Dec 8.
2
qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States.美国耐头孢他啶肠杆菌科分离株中qnr的流行情况。
Antimicrob Agents Chemother. 2006 Aug;50(8):2872-4. doi: 10.1128/AAC.01647-05.
3
Plasmid-mediated quinolone resistance in non-Typhi serotypes of Salmonella enterica.肠炎沙门氏菌非伤寒血清型中质粒介导的喹诺酮耐药性
Clin Infect Dis. 2006 Aug 1;43(3):297-304. doi: 10.1086/505397. Epub 2006 Jun 20.
4
Fluoroquinolone resistance and plasmid addiction systems: self-imposed selection pressure?氟喹诺酮耐药性与质粒成瘾系统：自我施加的选择压力？
J Antimicrob Chemother. 2006 Jun;57(6):1026-9. doi: 10.1093/jac/dkl110. Epub 2006 Apr 3.
5
Mutagenesis-based definitions and probes of residue burial in proteins.基于诱变的蛋白质中残基埋藏的定义与探测
Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16221-6. doi: 10.1073/pnas.0505089102. Epub 2005 Oct 26.
6
Molecular basis of gyrase poisoning by the addiction toxin CcdB.成瘾毒素CcdB导致回旋酶中毒的分子基础。
J Mol Biol. 2005 May 20;348(5):1091-102. doi: 10.1016/j.jmb.2005.03.049. Epub 2005 Apr 7.
7
Interaction of the plasmid-encoded quinolone resistance protein Qnr with Escherichia coli DNA gyrase.质粒编码的喹诺酮耐药蛋白Qnr与大肠杆菌DNA促旋酶的相互作用。
Antimicrob Agents Chemother. 2005 Jan;49(1):118-25. doi: 10.1128/AAC.49.1.118-125.2005.
8
ParE toxin encoded by the broad-host-range plasmid RK2 is an inhibitor of Escherichia coli gyrase.由广宿主范围质粒RK2编码的ParE毒素是大肠杆菌回旋酶的一种抑制剂。
Mol Microbiol. 2002 May;44(4):971-9. doi: 10.1046/j.1365-2958.2002.02921.x.
9
Mechanism of plasmid-mediated quinolone resistance.质粒介导的喹诺酮耐药机制。
Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5638-42. doi: 10.1073/pnas.082092899. Epub 2002 Apr 9.
10
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.由含有阿拉伯糖PBAD启动子的载体进行严格调控、调节和高水平表达。
J Bacteriol. 1995 Jul;177(14):4121-30. doi: 10.1128/jb.177.14.4121-4130.1995.