台湾槭（槭树科）的完整叶绿体基因组序列
The complete chloroplast genome sequence of Acer morrisonense (Aceraceae).
作者信息
Li Zhong-Hu, Xie Yu-Shan, Zhou Tao, Jia Yun, He Yan-Ling, Yang Jiao
机构信息
a Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education) , College of Life Sciences, Northwest University , Xi'an , China.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 May;28(3):309-310. doi: 10.3109/19401736.2015.1118091. Epub 2015 Dec 29.
Acer morrisonense (Aceraceae) is an important forest tree species and is endemic to Taiwan area. In this study, we determined the complete nucleotide sequence of A. morrisonense chloroplast genome (cpDNA) by using next-generation sequencing. The cpDNA was 157 197 bp in size, contained a pair of 26 728 bp inverted repeat (IR) regions, which were separated by a large single copy region (LSC) and a small single copy (SSC) region of 85 655 and 18 086 bp, respectively. The cpDNA contained 134 genes, including 86 protein-coding genes (78 PCG species), 8 ribosomal RNA genes (four rRNA species), and 40 transfer RNA genes (31 tRNA species). The most of gene species occur as a single copy, while 21 gene species occur in double copies. The overall AT content of A. morrisonense chloroplast genome were 62.2%, while the corresponding values of the LSC, SSC, and IR regions were 64.0%, 67.9%, and 57.3%, respectively. The phylogenetic analysis suggested that the cpDNA of A. morrisonense is closely related to that of congeneric A. buergerianum subsp. ningpoense.
台湾槭（槭树科）是一种重要的林木树种，为台湾地区特有。在本研究中，我们利用二代测序技术测定了台湾槭叶绿体基因组（cpDNA）的完整核苷酸序列。该cpDNA大小为157 197 bp，包含一对26 728 bp的反向重复（IR）区域，它们被一个85 655 bp的大单拷贝区域（LSC）和一个18 086 bp的小单拷贝（SSC）区域隔开。该cpDNA包含134个基因，其中包括86个蛋白质编码基因（78种PCG）、8个核糖体RNA基因（4种rRNA）和40个转运RNA基因（31种tRNA）。大多数基因种类以单拷贝形式存在，而21种基因以双拷贝形式存在。台湾槭叶绿体基因组的总体AT含量为62.2%，而LSC、SSC和IR区域的相应值分别为64.0%、67.9%和57.3%。系统发育分析表明，台湾槭的cpDNA与同属的宁波三角槭亚种的cpDNA密切相关。
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