Gao Ju, Gao Li-zhi
a Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , China and.
b Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, the Chinese Academy of Sciences , Kunming , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):983-5. doi: 10.3109/19401736.2014.926516. Epub 2014 Jun 18.
The complete nucleotide sequence of the Phyllostachys sulphurea chloroplast genome (cpDNA) was determined in this study. The cpDNA was 139,731 bp in length, containing a pair of 21,798 bp inverted repeat regions (IR), which were separated by small and large single copy regions (SSC and LSC) of 12,879 and 83,256 bp, respectively. The P. sulphurea cp genome encodes 129 predicted functional genes; 110 are unique (77 protein-coding genes, 29 tRNA genes, 4 rRNA), 19 are duplicated in the IR regions and one gene extended into the IR region in the junctions between IR and SSC. 43.06% of the genome sequence encodes proteins. The P. sulphurea cp genome is AT-rich (61.11%). In these genes, fourteen genes contained one intron, while one gene had two introns.
本研究测定了毛竹叶绿体基因组(cpDNA)的完整核苷酸序列。该cpDNA长度为139,731 bp,包含一对21,798 bp的反向重复序列(IR),它们被分别为12,879 bp和83,256 bp的小单拷贝区和大单拷贝区(SSC和LSC)隔开。毛竹cp基因组编码129个预测的功能基因;110个是独特的(77个蛋白质编码基因、29个tRNA基因、4个rRNA),19个在IR区域重复,一个基因在IR与SSC的交界处延伸到IR区域。基因组序列的43.06%编码蛋白质。毛竹cp基因组富含AT(61.11%)。在这些基因中,14个基因含有一个内含子,而一个基因有两个内含子。