来自可蛋白水解降解的透明质酸水凝胶的SDF-1α和BMP-2递送对骨修复的协同作用。
Synergistic Effects of SDF-1α and BMP-2 Delivery from Proteolytically Degradable Hyaluronic Acid Hydrogels for Bone Repair.
作者信息
Holloway Julianne L, Ma Henry, Rai Reena, Hankenson Kurt D, Burdick Jason A
机构信息
Department of Bioengineering, University of Pennsylvania, 210 S 33rd St, Philadelphia, Pennsylvania, 19104, USA.
Departments of Small Animal Clinical Sciences and Physiology, Michigan State University, 736 Wilson Rd, East Lansing, Michigan, 48824, USA.
出版信息
Macromol Biosci. 2015 Sep;15(9):1218-23. doi: 10.1002/mabi.201500178. Epub 2015 Jun 8.
Abstract
In order to achieve bone repair, bone morphogenetic protein-2 (BMP-2) is typically delivered in non-physiological doses and can result in significant adverse side effects. To reduce the amount of BMP-2 necessary for bone formation, we delivered a known chemokine (stromal cell derived factor-1α, SDF-1α) in combination with BMP-2 using proteolytically degradable hydrogels. A critical-sized calvarial defect was used to determine the effect of biomolecule delivery on bone formation in vivo. The treatment group with combined SDF-1α and BMP-2 hydrogel delivery showed significantly higher bone formation when compared to hydrogels loaded with the same BMP-2 or SDF-1α concentrations alone, suggesting the combined delivery of both biomolecules synergistically improves osteogenesis.
摘要
为了实现骨修复，骨形态发生蛋白-2（BMP-2）通常以非生理剂量递送，并且会导致显著的不良副作用。为了减少骨形成所需的BMP-2量，我们使用可蛋白水解降解的水凝胶将一种已知的趋化因子（基质细胞衍生因子-1α，SDF-1α）与BMP-2联合递送。使用临界尺寸的颅骨缺损来确定生物分子递送对体内骨形成的影响。与单独加载相同BMP-2或SDF-1α浓度的水凝胶相比，联合递送SDF-1α和BMP-2水凝胶的治疗组显示出显著更高的骨形成，这表明两种生物分子的联合递送协同改善了骨生成。
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