Marimón José María, Morales María, Cilla Gustavo, Vicente Diego, Pérez-Trallero Emilio
Microbiology Department, Hospital Universitario Donostia-IIS-Biodonostia, San Sebastián, Spain.
Biomedical Research Center Network for Respiratory Diseases (CIBERES), San Sebastián, Spain.
Future Microbiol. 2015;10(6):909-15. doi: 10.2217/fmb.14.143.
To study the etiology and the utility of new molecular methods in the diagnosis of complicated pneumonia with empyema.
MATERIALS & METHODS: Bacteria and viruses detection was performed by several traditional and molecular methods in the pleural fluid (PF) of 60 patients (38 children) with community-acquired pneumonia (CAP).
Despite prior antimicrobial therapy in 49 (81.7%) CAP patients, an etiological diagnosis could be established in 41 (68.3%), 35 being (58.3%) Streptococcus pneumoniae. PF culture was positive in only 6 patients but each molecular test detected more than 82% of cases.
Traditional culture methods have poor diagnostic sensitivity in PF because most CAP patients are under antimicrobial therapy when it is obtained. S. pneumoniae detection by molecular methods highly improves diagnosis.
研究复杂肺炎合并脓胸的病因及新型分子方法在其诊断中的应用价值。
采用多种传统及分子方法对60例（38例儿童）社区获得性肺炎（CAP）患者的胸腔积液（PF）进行细菌和病毒检测。
尽管49例（81.7%）CAP患者接受了抗菌治疗，但仍有41例（68.3%）可明确病因诊断，其中35例（58.3%）为肺炎链球菌。PF培养仅6例呈阳性，但每种分子检测方法检测出的病例均超过82%。
传统培养方法在PF中的诊断敏感性较差，因为大多数CAP患者在获取PF时已接受抗菌治疗。分子方法检测肺炎链球菌可显著提高诊断率。
