CK19、TPO及HBME-1表达对甲状腺乳头状癌诊断的意义
Significance of CK19, TPO, and HBME-1 expression for diagnosis of papillary thyroid carcinoma.
作者信息
Liu Zeming, Yu Pan, Xiong Yiquan, Zeng Wen, Li Xiaoyu, Maiaiti Yusufu, Wang Shuntao, Song Haiping, Shi Lan, Liu Chunping, Cheng Bo, Zhang Bo, Ming Jie, Dong Fang, Ge Hui, Nie Xiu, Huang Tao
机构信息
Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, Hubei, China.
出版信息
Int J Clin Exp Med. 2015 Mar 15;8(3):4369-74. eCollection 2015.
OBJECTIVES
To evaluate the expression and significance of CK19, TPO, and HBME-1 in the differential diagnosis of papillary thyroid carcinoma (PTC) and nonmalignant nodules.
METHODS
Tissue samples were obtained from 257 patients with PTC and 149 patients with nonmalignant thyroid specimens, and immunohistochemical staining for CK-19, TPO, and HBME-1 was performed.
RESULTS
The expression of CK-19, TPO, and HBME-1 was 96.3%, 12.0%, and 85.3%, respectively, for the PTC group. For nonmalignant thyroid lesions group, the expression of these markers was 40.4%, 86.2%, and 37.2%, respectively. Further, the expression of CK-19 and HBME-1 in PTCs was much higher than that in the benign thyroid lesions (P < 0.05). However, the positive expression of TPO in PTC specimens was much lower than that in the nonmalignant specimens (P < 0.05). CK-1 had the highest sensitivity (96.30%) for PTCs. The combination of the positive expression of CK-19 and negative expression of TPO had the highest sensitivity (98.50%), while that of the positive expression of HBME-1 and negative expression of TPO had the highest specificity (92.90%).
CONCLUSIONS
The combination of positive expression of CK-19 or HBME-1 or negative expression of TPO can improve the specificity of the diagnosis of PTC.
目的
评估细胞角蛋白19（CK19）、甲状腺过氧化物酶（TPO）和人甲状腺球蛋白单克隆抗体-1（HBME-1）在甲状腺乳头状癌（PTC）与非恶性结节鉴别诊断中的表达及意义。
方法
收集257例PTC患者及149例非恶性甲状腺标本患者的组织样本，进行CK-19、TPO和HBME-1免疫组化染色。
结果
PTC组中CK-19、TPO和HBME-1的表达率分别为96.3%、12.0%和85.3%。非恶性甲状腺病变组中，这些标志物的表达率分别为40.4%、86.2%和37.2%。此外，PTC中CK-19和HBME-1的表达远高于良性甲状腺病变（P<0.05）。然而，PTC标本中TPO的阳性表达远低于非恶性标本（P<0.05）。CK-1对PTC的敏感性最高（96.30%）。CK-19阳性表达与TPO阴性表达联合的敏感性最高（98.50%），而HBME-1阳性表达与TPO阴性表达联合的特异性最高（92.90%）。
结论
CK-19或HBME-1阳性表达或TPO阴性表达联合可提高PTC诊断的特异性。
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