Tomei L, Eap C B, Baumann P, Dente L
Dipartimento di Biochimica e Biotecnologie Mediche, II Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Hum Genet. 1989 Dec;84(1):89-91. doi: 10.1007/BF00210681.
Sera from transgenic mice (TM) carrying human genes of alpha 1-acid glycoprotein (orosomucoid or ORM) have been analyzed by isoelectrofocusing and subsequent immunoblotting with antihuman ORM antibodies. With this technique it is possible to reveal selectively the human protein secreted in the TM sera. Orosomucoid bands present in TM sera have been compared with those of the most common human ORM phenotypes to correlate the products of specific genes to previously identified genetic variants. In this paper, we report the identification of the genes encoding for variants ORM1 F1 and ORM2 A, which are genes AGP-A and AGP-B/B' respectively. The nucleotide sequences of these genes are known; therefore a direct correlation between variants and specific amino acid sequences can be established.
通过等电聚焦以及随后用抗人ORM抗体进行免疫印迹分析，对携带人α1-酸性糖蛋白（类粘蛋白或ORM）基因的转基因小鼠（TM）血清进行了分析。利用该技术能够选择性地揭示TM血清中分泌的人蛋白。已将TM血清中存在的类粘蛋白条带与最常见的人ORM表型的条带进行比较，以将特定基因的产物与先前鉴定的遗传变异相关联。在本文中，我们报告了编码变体ORM1 F1和ORM2 A的基因的鉴定，它们分别是AGP-A基因和AGP-B/B'基因。这些基因的核苷酸序列是已知的；因此，可以在变体与特定氨基酸序列之间建立直接关联。
