Lee Graham, Goosens Ki A
McGovern Institute for Brain Research, Massachusetts Institute of Technology.
McGovern Institute for Brain Research, Massachusetts Institute of Technology; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology;
J Vis Exp. 2015 May 18(99):e52766. doi: 10.3791/52766.
Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, proteins, and glucose, yet the composition of the blood is dynamically regulated and easily perturbed. One factor that can change the blood composition is the stress response triggered by the sampling procedure, which can contribute to variability in the measures of interest. Here we describe a procedure for blood sampling from the lateral tail vein in the rat. This procedure offers significant advantages over other more commonly used techniques. It permits rapid sampling with minimal pain or invasiveness, without anesthesia or analgesia. Additionally, it can be used to obtain large volume samples (upwards of 1 ml in some rats), and it may be used repeatedly across experimental days. By minimizing the stress response and pain resulting from blood sampling, measures can more accurately reflect the true basal state of the animal, with minimal influence from the sampling procedure itself.
在许多实验环境中，通常会采集血样以测量感兴趣的指标，包括激素、免疫因子、生长因子、蛋白质和葡萄糖等，但血液的成分是动态调节的，且容易受到干扰。采样过程引发的应激反应是能够改变血液成分的一个因素，它可能导致所测指标出现变异性。在此，我们描述一种从大鼠尾外侧静脉采集血样的方法。该方法相较于其他更常用的技术具有显著优势。它允许在几乎没有疼痛或侵入性的情况下快速采样，无需麻醉或镇痛。此外，它可用于获取大量样本（某些大鼠可达1毫升以上），并且可以在实验期间重复使用。通过将采血引起的应激反应和疼痛降至最低，所测指标能够更准确地反映动物的真实基础状态，采样过程本身的影响也会降至最低。
