Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570 752, South Korea.
PG & Research Department of Biotechnology, Mahendra Arts and Science College (Autonomous), Kalippatti 637 501, Namakkal, Tamil Nadu, India.
Chemosphere. 2015 Nov;138:127-32. doi: 10.1016/j.chemosphere.2015.05.059. Epub 2015 Jun 8.
The study was aimed to evaluate the potential of organic formulation, panchakavya, for enhancing the biological leaching of Pb and Cu in contaminated mine soil. Response surface methodology based Box-Behnken design was used to optimize the variables such as incubation time, panchakavya concentration, and agitation rate. The maximum bioleaching (Pb=64% and Cu=49%) was observed after 54 h of incubation with 10 mL panchakavya at 120 rpm. Statistics-based contour and three-dimensional plots were generated to understand the relationship between Pb and Cu bioleaching and variables. High-performance liquid chromatography analysis showed the presence of lactic (25.88 mg g(-1)), citric (0.14 mg g(-1)), succinic (0.14 mg g(-1)), malic (0.66 mg g(-1)), and acetic (0.44 mg g(-1)) acids in panchakavya, which may have a vital role in the removal of metals from the contaminated soil. Soil fraction studies indicate a significant increase of Pb (45%) in the exchangeable fraction of panchakavya-treated soil. XRD studies confirmed the role of panchakavya induced calcite and other minerals in the precipitation of metal ions. A significant increase in the enzyme activities of phosphatase, dehydrogenase, urease, amylase, invertase, and cellulase were observed in the panchakavya-treated soil.
本研究旨在评估有机配方（panchakavya）在增强污染矿山土壤中 Pb 和 Cu 的生物浸出方面的潜力。采用基于响应面法的 Box-Behnken 设计来优化变量，如孵育时间、panchakavya 浓度和搅拌速度。在 120 rpm 下孵育 54 小时，用 10 mL panchakavya 处理后，观察到最大生物浸出率（Pb=64%和 Cu=49%）。基于统计的等高线和三维图生成，以了解 Pb 和 Cu 生物浸出与变量之间的关系。高效液相色谱分析表明，panchakavya 中存在乳酸（25.88 mg g(-1)）、柠檬酸（0.14 mg g(-1)）、琥珀酸（0.14 mg g(-1)）、苹果酸（0.66 mg g(-1)）和乙酸（0.44 mg g(-1)），这些酸可能在从污染土壤中去除金属方面发挥重要作用。土壤分馏研究表明，panchakavya 处理土壤中 Pb（45%）在可交换态的比例显著增加。XRD 研究证实了 panchakavya 诱导的方解石和其他矿物质在金属离子沉淀中的作用。在 panchakavya 处理的土壤中，磷酸酶、脱氢酶、脲酶、淀粉酶、转化酶和纤维素酶的酶活性显著增加。
