迈向使用环境敏感黄酮传感器在SDS-PAGE上简化血清白蛋白检测的一步。
A step toward simplified detection of serum albumin on SDS-PAGE using an environment-sensitive flavone sensor.
作者信息
Liu Bin, Pang Yi, Bouhenni Rachida, Duah Ernest, Paruchuri Sailaja, McDonald Lucas
机构信息
Department of Chemistry, University of Akron, Akron, Ohio 44325, USA.
出版信息
Chem Commun (Camb). 2015 Jul 14;51(55):11060-3. doi: 10.1039/c5cc03516c.
Abstract
In this study, we report a series of novel flavone-based sensors that exhibit a superior fluorescence response when interacting with serum albumin in real serum samples and in acrylamide gels. The detection limit of probe 4 for serum albumin solution is 0.09 μg mL(-1), and the detectable volume for monkey serum reaches as low as 0.03 μL.
摘要
在本研究中，我们报道了一系列新型黄酮类传感器，它们在实际血清样本和丙烯酰胺凝胶中与血清白蛋白相互作用时表现出优异的荧光响应。探针4对血清白蛋白溶液的检测限为0.09 μg mL(-1)，对猴血清的可检测体积低至0.03 μL。
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