Zhao Yi-Jun, Han Hua-Zhong, Liang Yong, Shi Chen-Zhang, Zhu Qing-Chao, Yang Jun
Yi-Jun Zhao, Hua-Zhong Han, Chen-Zhang Shi, Qing-Chao Zhu, Jun Yang, Department of Surgery, Shanghai Jiao Tong University, Affiliated Sixth People's Hospital, Shanghai 200233, China.
World J Gastroenterol. 2015 Jun 7;21(21):6550-60. doi: 10.3748/wjg.v21.i21.6550.
To investigate alternative splicing in vascular endothelial growth factor A (VEGFA), amyloid beta precursor protein (APP), and Numb homolog (NUMB) in colorectal cancer (CRC).
Real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and PCR-restriction fragment length polymorphism analyses were performed to detect the expression of VEGFA, APP, and NUMB mRNA in 20 CRC tissues and matched adjacent normal tissues, as well as their alternative splicing variants.
qRT-PCR analysis revealed that the expression of APP, NUMB, and VEGFA165b mRNA were significantly downregulated, while VEGFA mRNA was upregulated, in CRC tissues (all P < 0.05). PCR-restriction fragment length polymorphism analysis revealed that the expression of VEGFA165a/b in CRC tissues was significantly higher than in adjacent normal tissues (P < 0.05). Compared with adjacent normal tissues, the expression of NUMB-PRR(S) in CRC tissues was significantly decreased (P < 0.05), and the expression of NUMB-PRR(L) was increased (P < 0.05).
Alternative splicing of VEGFA, APP, and NUMB may regulate the development of CRC, and represent new targets for its diagnosis, prognosis, and treatment.
研究结直肠癌（CRC）中血管内皮生长因子A（VEGFA）、淀粉样前体蛋白（APP）和Numb同源物（NUMB）的可变剪接。
采用实时定量逆转录聚合酶链反应（qRT-PCR）和PCR-限制性片段长度多态性分析，检测20例CRC组织及其配对的癌旁正常组织中VEGFA、APP和NUMB mRNA的表达及其可变剪接变体。
qRT-PCR分析显示，CRC组织中APP mRNA、NUMB mRNA和VEGFA165b mRNA表达显著下调，而VEGFA mRNA表达上调（均P<0.05）。PCR-限制性片段长度多态性分析显示，CRC组织中VEGFA165a/b的表达显著高于癌旁正常组织（P<0.05）。与癌旁正常组织相比，CRC组织中NUMB-PRR(S)表达显著降低（P<0.05），NUMB-PRR(L)表达升高（P<0.05）。
VEGFA、APP和NUMB的可变剪接可能调控CRC的发生发展，有望成为CRC诊断、预后及治疗的新靶点。
