Birnstiel M L, Kressmann A, Schaffner W, Portmann R, Busslinger M
Philos Trans R Soc Lond B Biol Sci. 1978 May 11;283(997):319-24. doi: 10.1098/rstb.1978.0031.
Sequencing of cloned histone DNA of the sea urchin Psammechinus miliaris has confirmed the map of the histone genes obtained earlier by rather less refined techniques. Sequencing of spacer has revealed that it is unlikely to code for protein. Some interesting sequences in the prelude regions to the structural genes have been found. The technique of injecting DNA into the germinal vesicle of the Xenopus oocyte has been greatly simplified, so that now many of the parameters governing the transcription of the injected genes can be investigated. Some mRNA-like molecules appear when circular histone DNA is inserted into the oocyte nucleus. We are cautiously optimistic that the technique can be further developed and will provide a useful tool for the study of the molecular mechanisms governing the expression of structural genes coding for proteins.
对海胆光棘球海胆克隆的组蛋白DNA进行测序，证实了先前通过不太精细的技术获得的组蛋白基因图谱。间隔区的测序表明它不太可能编码蛋白质。在结构基因的前奏区域发现了一些有趣的序列。将DNA注入非洲爪蟾卵母细胞生发泡的技术已大大简化，因此现在可以研究许多控制注入基因转录的参数。当环状组蛋白DNA插入卵母细胞核时会出现一些类似mRNA的分子。我们谨慎地乐观地认为，该技术可以进一步发展，并将为研究编码蛋白质的结构基因表达的分子机制提供有用的工具。
