Birnstiel M L, Schaffner W, Smith H O
Nature. 1977 Apr 14;266(5603):603-7. doi: 10.1038/266603a0.
Starting from restriction enzyme cleavage sites of known topologies the DNA sequences coding for two thirds of the H2B histone protein, together with some 3 inch extracistronic sequences, have been determined for the sea urchin Psammechinus miliaris. This unambiguously identifies this gene and further reveals its 5 inch-3inch polarity and accurate map position in a cloned histone DNA repeat unit.
从已知拓扑结构的限制性内切酶切割位点出发，已经确定了海胆粗疣海胆编码三分之二H2B组蛋白的DNA序列，以及大约3英寸的顺反子外序列。这明确地鉴定了该基因，并进一步揭示了其5英寸-3英寸的极性以及在克隆的组蛋白DNA重复单元中的精确图谱位置。
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