Jiang Pinliang, Liang Jianhe, Song Ran, Zhang Yanmei, Ren Lei, Zhang Lihai, Tang Peifu, Lin Changjian
§Department of Orthopaedics, General Hospital of Chinese PLA, Beijing 100853, China.
ACS Appl Mater Interfaces. 2015 Jul 8;7(26):14384-96. doi: 10.1021/acsami.5b03172. Epub 2015 Jun 29.
Surface structures and properties of titanium implants play a vital role in successful bone replacement. To mimic the natural bone structure, some strategies have recently focused on the preparation of multiscaled morphology on medical titanium and shown some promising results; however, relatively few efforts have been made for further enhancing the biocompatibility of such a hierarchical hybrid structure without compromising the superior bioactivity of the starting micro/nano roughness. In this study, a thin ribbonlike octacalcium phosphate (OCP) coating was electrodeposited on a hierarchically structured titania surface, maintaining its micro/nanospongelike morphology. It is indicated that the micro/nanostructured surface with deposited OCP showed an improved biomineralization ability, in comparison to that without OCP modification, when immersed in simulated body fluid (SBF). Further evaluations of cellular activities demonstrated that the introduction of OCP to the micro/nano spongelike-structured surface remarkably enhanced MC3T3-E1 cell proliferation, alkaline phosphatase activity, and extracellular matrix mineralization compared to that of cells on the micro/nanospongelike titania surface during 14 days of culturing. Meanwhile, the OCP-deposited micro/nanostructured surface displayed much a smaller passive current density and lower current response to the applied potential, resulting in the improvement of corrosion resistance. All of the evaluations suggested that the modification of the OCP coating on the prepared micro/nanospongelike titania is of superior chemical stability, biomineralization, and osteoblast activities, which indicates a favorable implant microenvironment for osseointegration in vivo.
钛植入物的表面结构和性能对成功的骨替代起着至关重要的作用。为了模拟天然骨结构，最近一些策略聚焦于在医用钛上制备多尺度形态，并取得了一些有前景的结果；然而，在不损害起始微/纳米粗糙度的优异生物活性的前提下，为进一步提高这种分级混合结构的生物相容性所做的努力相对较少。在本研究中，在具有分级结构的二氧化钛表面电沉积了一层薄的带状磷酸八钙（OCP）涂层，同时保持其微/纳米海绵状形态。结果表明，与未进行OCP改性的表面相比，沉积有OCP的微/纳米结构表面在浸入模拟体液（SBF）时表现出更高的生物矿化能力。对细胞活性的进一步评估表明，在14天的培养过程中，与微/纳米海绵状二氧化钛表面上的细胞相比，在微/纳米海绵状结构表面引入OCP显著增强了MC3T3-E1细胞的增殖、碱性磷酸酶活性和细胞外基质矿化。同时，沉积有OCP的微/纳米结构表面显示出小得多的被动电流密度和对施加电位的较低电流响应，从而提高了耐腐蚀性。所有评估表明，在制备的微/纳米海绵状二氧化钛上修饰OCP涂层具有优异的化学稳定性、生物矿化能力和成骨细胞活性，这表明在体内为骨整合提供了良好的植入微环境。
