†Department of Animal Science, Aarhus University, AU-Foulum, Blichers Alle 20, P.O. Box 50, DK-8830 Tjele, Denmark.
§Danish Cancer Society Research Center, Strandboulevarden 49, DK-2100 Copenhagen, Denmark.
J Agric Food Chem. 2015 Jul 15;63(27):6283-92. doi: 10.1021/acs.jafc.5b01275. Epub 2015 Jul 2.
Lignans have gained nutritional interest due to their promising role in the prevention of lifestyle diseases. However, epidemiological studies are in need of more evidence to link the intake of lignans to this promising role. In this context, it is necessary to study large population groups to obtain sufficient statistical power. Therefore, there is a demand for fast, sensitive, and accurate methods for quantitation with high throughput of samples. This paper presents a validated LC-MS/MS method for the quantitation of eight plant lignans (matairesinol, hydroxymatairesinol, secoisolariciresinol, lariciresinol, isolariciresinol, syringaresinol, medioresinol, and pinoresinol) and two enterolignans (enterodiol and enterolactone) in both human and pig plasma and urine. The method showed high selectivity and sensitivity allowing quantitation of lignans in the range of 0.024-100 ng/mL and with a run time of only 4.8 min per sample. The method was successfully applied to quantitate lignans in biofluids from ongoing studies with humans and pigs.
木脂素因其在预防生活方式疾病方面的潜力而引起了人们的营养兴趣。然而，流行病学研究需要更多的证据来将木脂素的摄入量与这一有希望的作用联系起来。在这种情况下，有必要研究大型人群群体，以获得足够的统计能力。因此，需要快速、灵敏和准确的方法来进行高通量的样品定量分析。本文介绍了一种经验证的 LC-MS/MS 方法，用于定量测定人血浆和尿液中的八种植物木脂素（马替瑞林、羟基马替瑞林、开环异落叶松脂醇、松脂醇、异松脂醇、丁香树脂醇、二甲氧基树脂醇和橄榄树脂醇）和两种肠内木脂素（肠二醇和肠内酯）。该方法具有很高的选择性和灵敏度，允许在 0.024-100ng/mL 的范围内定量分析木脂素，每个样品的运行时间仅为 4.8 分钟。该方法成功地应用于正在进行的人类和猪的生物流体中木脂素的定量分析。
