Sweet S P, MacFarlane T W, Samaranayake L P
Department of Oral Medicine and Pathology, University of Glasgow, Dental Hospital and School, Scotland.
Microbios. 1989;60(242):15-22.
Exfoliated buccal epithelial cells have been widely used in microbial adherence studies, but present a number of problems due to their variable nature and to contamination with indigenous bacteria. An adherence assay was developed using HeLa cell monolayers which were washed with buffer, or treated with saliva or serum to mimic buccal or crevicular epithelial cells, respectively. A total of eighteen strains of oral bacteria tested showed a low affinity for untreated HeLa cells, but most strains adhered in high numbers to saliva treated HeLa cells. A few strains, usually present in the gingival crevice, demonstrated a high affinity for serum treated HeLa cells. Thus, salivary and crevicular fluid components appear to be specifically implicated in the selective adherence and colonization of bacteria on oral surfaces.
脱落的颊上皮细胞已广泛用于微生物黏附研究，但由于其性质多变以及受本土细菌污染而存在一些问题。我们开发了一种黏附试验，使用HeLa细胞单层，分别用缓冲液洗涤，或用唾液或血清处理，以分别模拟颊上皮细胞或龈沟上皮细胞。总共测试的18株口腔细菌对未处理的HeLa细胞亲和力较低，但大多数菌株大量黏附于经唾液处理的HeLa细胞。一些通常存在于牙龈沟中的菌株对经血清处理的HeLa细胞表现出高亲和力。因此，唾液和龈沟液成分似乎特别参与了细菌在口腔表面的选择性黏附和定植。
