用于经导管递送至肝肿瘤的MRI的自然杀伤细胞的临床适用磁性标记：临床转化的临床前验证
Clinically applicable magnetic-labeling of natural killer cells for MRI of transcatheter delivery to liver tumors: preclinical validation for clinical translation.
作者信息
Li Kangan, Gordon Andrew C, Zheng Linfeng, Li Weiguo, Guo Yang, Sun Jing, Zhang Guixiang, Han Guohong, Larson Andrew C, Zhang Zhuoli
机构信息
Department of Radiology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P. R. China.
Departments of Radiology, Northwestern University, 737 N Michigan Ave, Suite 1600, Chicago, IL 60611, USA.
出版信息
Nanomedicine (Lond). 2015;10(11):1761-74. doi: 10.2217/nnm.15.24.
Abstract
AIM
To test the hypothesis that MRI can monitor intraportal vein (IPV) transcatheter delivery of clinically applicable heparin-protamine-ferumoxytol (HPF) nanocomplex-labeled natural killer (NK) cells to liver tumor.
MATERIALS & METHODS: Liver tumor rat models underwent catheterization for IPV infusion of HPF-labeled NK cells (NK-92MI cell line). MRI measurements within tumor and adjacent liver tissues were compared pre- and post-NK cell infusion. Histology studies were used to identify NK cells in the target tumors.
RESULTS
For first time, we demonstrated that MRI tracks HPF-labeled NK cells migration within liver following IPV delivery.
CONCLUSION
IPV transcatheter infusion permitted selective delivery of NK cells to liver tissues and MRI allowed tracking NK cell biodistributions within the tumors.
摘要
目的
验证以下假设，即磁共振成像（MRI）能够监测经门静脉（IPV）导管输送临床上适用的肝素-鱼精蛋白-菲立磁（HPF）纳米复合物标记的自然杀伤（NK）细胞至肝肿瘤的情况。
材料与方法
肝肿瘤大鼠模型接受导管插入术，以便经门静脉输注HPF标记的NK细胞（NK-92MI细胞系）。在输注NK细胞前后，对肿瘤及相邻肝组织进行MRI测量并比较。组织学研究用于识别靶肿瘤中的NK细胞。
结果
我们首次证明，MRI能够追踪经门静脉输送后HPF标记的NK细胞在肝脏内的迁移情况。
结论
经门静脉导管输注可使NK细胞选择性地输送至肝组织，而MRI能够追踪NK细胞在肿瘤内的生物分布情况。
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Clin Cancer Res. 2014 Jul 1;20(13):3390-400. doi: 10.1158/1078-0432.CCR-13-1766.
2
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Stem Cells Transl Med. 2013 Oct;2(10):766-75. doi: 10.5966/sctm.2013-0049. Epub 2013 Sep 6.
3
Image-guided local delivery strategies enhance therapeutic nanoparticle uptake in solid tumors.影像引导局部递药策略增强实体瘤中治疗性纳米颗粒的摄取。
ACS Nano. 2013 Sep 24;7(9):7724-33. doi: 10.1021/nn4023119. Epub 2013 Aug 20.
4
NK cell-based immunotherapy for malignant diseases.基于自然杀伤细胞的恶性疾病免疫疗法。
Cell Mol Immunol. 2013 May;10(3):230-52. doi: 10.1038/cmi.2013.10. Epub 2013 Apr 22.
5
Focused ultrasound delivers targeted immune cells to metastatic brain tumors.聚焦超声将靶向免疫细胞递送到转移性脑肿瘤。
Cancer Res. 2013 Mar 15;73(6):1892-9. doi: 10.1158/0008-5472.CAN-12-2609. Epub 2013 Jan 9.
6
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8
Variations in feeding arteries of hepatocellular carcinoma located in the left hepatic lobe.左肝叶肝癌供血动脉的变异。
Jpn J Radiol. 2012 Jul;30(6):471-9. doi: 10.1007/s11604-012-0075-6. Epub 2012 Apr 3.
9
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