Chen Xiaomei, Li Xiangping, Zhao Feipeng, Huang Haoran, Lu Juan, Liu Xiong
Department of Otorhinolaryngology,Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Department of Otorhinolaryngology,Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Email:
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2015 Apr;50(4):306-11.
To investigate the infiltration and prognostic significance of tumor-infiltrating mast cells (TIMs) in nasopharyngeal carcinoma (NPC).
Immunohistochemistry for tryptase was performed on 154 NPC specimens. The median value of TIM density was used as a cutoff point to separate the patient cohort into two groups with either low or high TIM infiltration. The associations between TIM and clinicopathological factors were analyzed by Mann-Whitney U text. Survival curves were plotted according to the Kaplan-Meier method. Univariate and multivariate analyses were performed using the Log-rank test and the Cox proportional hazard models, respectively. P<0.05 was considered statistically significant. All statistical analyses were conducted using SPSS 13.0.
TIM was mainly in the stroma of NPC and detected in all specimens. The median value of TIM density (25.60/high power field) was used as a cutoff point to separate the patient cohort into two groups with either low or high TIM infiltration. The density of TIM was positively correlated with N stage (Z=-2.193, P<0.05) and clinical stage (Z=-2.551, P<0.05). The 3-year overall survival (OS) and progression-free survival (PFS) of patients were 64.4% and 55.7% in the high TIM density group; 78.3% and 77.0% in the low TIM density group. For survival evaluation, high density of TIM was associated with worse OS and PFS (P<0.05). Multivariate Cox regression model analysis showed TIM infiltration was an independent risk factor for both OS and PFS.
The density of TIM in NPC increased with tumor stage. High TIM infiltration was associated with poor overall survival and progression-free survival.
探讨肿瘤浸润肥大细胞（TIMs）在鼻咽癌（NPC）中的浸润情况及其预后意义。
对154例NPC标本进行色氨酸酶免疫组化检测。以TIM密度的中位数作为分界点，将患者队列分为TIM浸润低或高的两组。采用Mann-Whitney U检验分析TIM与临床病理因素之间的关联。根据Kaplan-Meier法绘制生存曲线。分别使用Log-rank检验和Cox比例风险模型进行单因素和多因素分析。P<0.05被认为具有统计学意义。所有统计分析均使用SPSS 13.0进行。
TIM主要存在于NPC的基质中，所有标本均能检测到。以TIM密度的中位数（25.60/高倍视野）作为分界点，将患者队列分为TIM浸润低或高的两组。TIM密度与N分期（Z=-2.193，P<0.05）和临床分期（Z=-2.551，P<0.05）呈正相关。高TIM密度组患者的3年总生存率（OS）和无进展生存率（PFS）分别为64.4%和55.7%；低TIM密度组分别为78.3%和77.0%。在生存评估中，高TIM密度与较差的OS和PFS相关（P<0.05）。多因素Cox回归模型分析显示，TIM浸润是OS和PFS的独立危险因素。
NPC中TIM的密度随肿瘤分期增加。高TIM浸润与较差的总生存和无进展生存相关。
