携带7,8-二氢-8-氧代腺嘌呤的DNA片段的合成：一种γ辐射产物。
Synthesis of DNA fragments bearing 7,8-dihydro-8-oxo-adenine: a gamma radiation product.
作者信息
Guy A, Molko D, Téoule R
机构信息
Département de Recherche Fondamentale, Centre d'Etudes Nucléaires de Grenoble, France.
出版信息
Nucleic Acids Symp Ser. 1989(21):135-6.
PMID:2608463
Abstract
In order to investigate the mutagenic effects and the repair of DNA lesions induced by gamma rays, 7,8-dihydro-8-oxo-adenine was introduced in DNA fragments using cyanoethyl phosphoroamidite method. The modified oligonucleotides were purified by HPLC and the presence of the defect in the final product was confirmed by DNA sequencing, enzymatic hydrolysis and FAB mass spectrometry.
摘要
为了研究γ射线诱导的DNA损伤的诱变效应及其修复，采用氰乙基亚磷酰胺法将7,8-二氢-8-氧代腺嘌呤引入DNA片段中。修饰后的寡核苷酸通过高效液相色谱法进行纯化，并通过DNA测序、酶促水解和快原子轰击质谱法确认最终产物中缺陷的存在。
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