Moran L, Mirault M E, Arrigo A P, Goldschmidt-Clermont M, Tissières A
Philos Trans R Soc Lond B Biol Sci. 1978 May 11;283(997):391-406. doi: 10.1098/rstb.1978.0044.
The heat shock proteins, labelled in vivo with [35S]methionine, were separated by sodium dodecylsulphate-polyacrylamide gel electrophoresis and fingerprinted after tryptic digestion. Eight distinct heat shock polypeptides are characterized in this way. Heat shock messenger RNAs were isolated and partially purified. Assayed in vitro for protein synthesis, they were found to code for heat shock polypeptides. Some parameters of the kinetics of in vivo synthesis of the heat shock proteins are presented.
用[35S]甲硫氨酸在体内标记的热休克蛋白，通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳进行分离，并在胰蛋白酶消化后进行指纹分析。通过这种方式鉴定出了8种不同的热休克多肽。分离并部分纯化了热休克信使核糖核酸。在体外进行蛋白质合成测定时，发现它们编码热休克多肽。本文还介绍了热休克蛋白体内合成动力学的一些参数。
