用于快速灵敏鉴定多动物隐秘杆菌的环介导等温扩增（LAMP）检测方法的开发。
Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium.
作者信息
Abdulmawjood A, Wickhorst J, Sammra O, Lämmler C, Foster G, Wragg P N, Prenger-Berninghoff E, Klein G
机构信息
Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
出版信息
Mol Cell Probes. 2015 Dec;29(6):468-472. doi: 10.1016/j.mcp.2015.06.003. Epub 2015 Jun 18.
In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment.
在本研究中，从不同来源分离出的28株多宿主隐秘杆菌菌株能够通过基于编码多宿主溶菌素的pla基因新设计的环介导等温扩增（LAMP）试验成功鉴定。代表隐秘杆菌属5个种和特鲁珀菌属5个种的对照菌株未观察到类似反应。所展示的pla LAMP试验可能也能让设备不太精良的实验室可靠且低成本地鉴定这种细菌病原体。
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