一种环介导等温扩增（LAMP）检测方法在对从不同来源分离的化脓放线杆菌进行分子鉴定中的应用。
Application of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella pyogenes isolated from various origins.
作者信息
Abdulmawjood A, Wickhorst J, Hashim O, Sammra O, Hassan A A, Alssahen M, Lämmler C, Prenger-Berninghoff E, Klein G
机构信息
Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
出版信息
Mol Cell Probes. 2016 Aug;30(4):205-210. doi: 10.1016/j.mcp.2016.05.003. Epub 2016 May 27.
In the present study 28 Trueperella pyogenes strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene cpn60 encoding chaperonin. No cross reaction could be observed with control strains representing four species of genus Trueperella and seven species of closely related genus Arcanobacterium. The present cpn60 LAMP assay might allow a reliable and low cost identification of T. pyogenes also in laboratories with less specified equipment.
在本研究中，从不同来源分离出的28株化脓放线杆菌菌株能够通过一种基于编码伴侣蛋白的cpn60基因新设计的环介导等温扩增（LAMP）检测法成功鉴定。与代表放线杆菌属4个种和密切相关的隐秘杆菌属7个种的对照菌株未观察到交叉反应。目前的cpn60 LAMP检测法可能也允许在设备不太精良的实验室中对化脓放线杆菌进行可靠且低成本的鉴定。
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