He Yanyu, Luo Yuzhu, Cheng Lixiang, Wang Jiqing, Liu Xiu, Shao Bin, Cui Yan
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China.
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou, Gansu, China.
Anat Rec (Hoboken). 2015 Oct;298(10):1796-803. doi: 10.1002/ar.23185. Epub 2015 Jul 23.
This experiment was conducted to identify some aspects of secondary follicle (SF) characteristics of Hexi cashmere goats at five different growing stages in one year in order and discover the expression pattern of Hoxc13 in a SF cycling to provide morphological basis for studying the growth of cashmere. Ten cashmere goats of one-year old (5 males, 5 females) were included in this study. The density and activity of SF were observed and measured by making paraffin sections, the ultra-structural features of SF were studied under transmission electron microscopy (TEM) by making ultra-thin sections, and the expression of Hoxc13 was investigated through the immunohistochemistry method. The average diameter of SF had the smallest value in the anagen stage, and significant difference (P < 0.05) was found between the anagen stage and other stages. The density of SF increased gradually through the different growing stages, and significant difference was found between the anagen and procatagen stages (P < 0.05). With an increase in time (months), the percentage of SF activity increased, and significant difference in the percentage of SF activity was found between the telogen and anagen stages (P < 0.05). At the telogen stage, the layers of connective tissue sheath (CTS) of SF were unclear, hemidesmosomes between the outer root sheath (ORS) and basement membrane disappeared, and dead cells were found at the top of the SF. The rudiments of new SF were found in the proanagen stage, CTS was thickened, and the cells of ORS were stretched out like fingers. At the anagen stage, the structure of SF was integral, and the inner root sheath (IRS) consisted of three concentric layers (Helen, Huxley, and Cuticle layers). The cells of Huxley's layer degenerated gradually, and pseudopodia were formed on the cells of ORS in the procatagen stage. At the catagen stage, the ORS was separated from the IRS, and IRS disappeared. Huxley's layer was absent in the inactive SF while, the ORS was present in the active SF. Hoxc 13 was expressed in the epidermis and sebaceous gland o, ORS, IRS, hair shaft of SF in the skin. Hoxc13 was expressed weakly during procatagen, catagen, and telogen stage, while with an increase in proanagen and anagen stage, significant difference was found between them. These findings demonstrated the ultra-structural features of SF could provide the useful activity criteria, and Hoxc13 associated with the SF activity.
本实验旨在依次确定河西绒山羊在一年中五个不同生长阶段的次级毛囊（SF）特征的某些方面，并发现Hoxc13在SF周期中的表达模式，为研究羊绒生长提供形态学依据。本研究纳入了10只一岁的绒山羊（5只雄性，5只雌性）。通过制作石蜡切片观察和测量SF的密度和活性，通过制作超薄切片在透射电子显微镜（TEM）下研究SF的超微结构特征，并通过免疫组织化学方法研究Hoxc13的表达。SF的平均直径在生长期最小，生长期与其他阶段之间存在显著差异（P < 0.05）。SF的密度在不同生长阶段逐渐增加，生长期与生长前期之间存在显著差异（P < 0.05）。随着时间（月）的增加，SF活性百分比增加，休止期与生长期之间的SF活性百分比存在显著差异（P < 0.05）。在休止期，SF的结缔组织鞘（CTS）层不清晰，外根鞘（ORS）与基底膜之间的半桥粒消失，SF顶部发现死细胞。在生长前期发现了新SF的原基，CTS增厚，ORS细胞像手指一样伸展。在生长期，SF结构完整，内根鞘（IRS）由三个同心层（亨勒层、赫胥黎层和角质层）组成。赫胥黎层的细胞逐渐退化，在退行期ORS细胞上形成伪足。在退化期，ORS与IRS分离，IRS消失。静止的SF中没有赫胥黎层，而活跃的SF中有ORS。Hoxc13在皮肤中SF的表皮、皮脂腺、ORS、IRS和毛干中表达。Hoxc13在退行期、退化期和休止期表达较弱，而随着生长前期和生长期的增加，它们之间存在显著差异。这些发现表明，SF的超微结构特征可以提供有用的活性标准，并且Hoxc13与SF活性相关。
