Wang Jiqing, Yu Ping, Wang Haifang, He Yanyu
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Department of Otorhinolaryngology, Second Hospital Affiliated to Lanzhou University, Lanzhou, China.
Anat Rec (Hoboken). 2018 Apr;301(4):742-752. doi: 10.1002/ar.23724. Epub 2017 Nov 28.
This experiment was conducted to identify periodic changes in secondary follicles (SFs) over one year of extensive (grazed) and intensive (housed and fed supplement) farming of Longdong cashmere goats. One-year old goats (n = 24, 12 extensively fed and 12 intensively fed) were studied. The diameter, depth, density, activity, and ultra-structural features of SFs were assessed using light microscopy and transmission electron microscopy. HOXC13 and HSP27 expression were studied using immunohistochemistry and immunofluorescence method. The anagen stage in the extensively grazed goats was from April to September, but was longer (April to October) in the housed, supplementary fed group. The depth and activity of the SF in anagen and catagen differed (P < 0.05) between the groups. HSP27 and HOXC13 protein were present in both the epidermis and dermis, with HSP27 immunoreactivity highest in the hair shaft (HS), outer root sheath (ORS) and inner root sheath (IRS). HOXC13 expression was prominent in both the eprdermis and ORS. HSP27 and HOXC13 expression were prominent during anagen, and less so during catagen and telogen. In anagen, HSP27 expression in the HS, IRS, and ORS of the extensively fed group was higher than in the intensively fed group (p < 0.05). In contrast, HOXC13 expression in HS, IRS and ORS of the extensively fed group was lower than in the intensively fed group (P < 0.05). This suggests the growth of cashmere is influenced by nutrition and that housed goats could be used in cashmere production. Anat Rec, 301:742-752, 2018. © 2017 Wiley Periodicals, Inc.
本实验旨在确定陇东绒山羊在一年的粗放（放牧）和集约（圈养并补饲）养殖过程中次级毛囊（SFs）的周期性变化。研究对象为1岁山羊（n = 24，其中12只粗放饲养，12只集约饲养）。使用光学显微镜和透射电子显微镜评估SFs的直径、深度、密度、活性和超微结构特征。采用免疫组织化学和免疫荧光法研究HOXC13和HSP27的表达。粗放放牧山羊的生长期为4月至9月，但圈养补饲组的生长期更长（4月至10月）。两组之间生长期和退行期的SF深度和活性存在差异（P < 0.05）。HSP27和HOXC13蛋白在表皮和真皮中均有表达，其中HSP27免疫反应性在毛干（HS）、外根鞘（ORS）和内根鞘（IRS）中最高。HOXC13在表皮和ORS中均有明显表达。HSP27和HOXC13在生长期表达明显，在退行期和休止期表达较少。在生长期，粗放饲养组的HS、IRS和ORS中HSP27的表达高于集约饲养组（p < 0.05）。相反，粗放饲养组的HS、IRS和ORS中HOXC13的表达低于集约饲养组（P < 0.05）。这表明羊绒生长受营养影响，圈养山羊可用于羊绒生产。《解剖学记录》，301:742 - 752，2018年。© 2017威利期刊公司。
