College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, Hohhot, China.
PLoS One. 2018 Oct 24;13(10):e0204404. doi: 10.1371/journal.pone.0204404. eCollection 2018.
There are two main types of hair follicle in Inner Mongolia Cashmere goats, the primary hair follicle (PHF) producing hair fibers and the secondary hair follicle (SHF) producing cashmere fibers. Of both fibers from cashmere-bearing goats in Aerbasi, Inner Mongolia, the timing of cyclical phases for the cashmere have been well clarified but hair fibers have been less noticeable. Herein, we evaluated transcriptome of PHF and SHF from the same three goats in Aerbasi at the catagen- and telogen phase of cashmere growth. We totally found 1977 DEGs between PHFs at the telogen and catagen phases of SHF, 1199 DEGs between telogen- and catagen SHF, 2629 DEGs between PHF at the catagen phase of SHF and catagen SHF, and 755 DEGs between PHF at the telogen phase of SHF and telogen SHF. By analyzing gene functions based on GO and KEGG database, we found that the DEGs have functions in muscle contraction and muscle filament sliding between catagen- and telogen SHF, indicating that arrector pilli muscles might play a role on the transition from catagen to telogen. Moreover, considering that the enriched GO and KEGG categories of the DEGs between PHF and SHF, we suggested that part of PHF might rest in their own anagen phase when SHF are at catagen, but PHF might enter into the telogen phase at SHF's telogen. Finally, we highly recommended the several potential genes acting as the regulators of the transition between growth phases including IL17RB and eight members of ZNF. These results provide insight into molecular mechanisms on the transition of SHF from catagen to telogen together with PHF's growth situation at SHF's catagen and telogen in Inner Mongolia Cashmere goats.
内蒙古绒山羊有两种主要类型的毛囊，初级毛囊（PHF）产生毛发纤维，次级毛囊（SHF）产生羊绒纤维。在内蒙古阿尔巴斯绒山羊的有绒和无绒个体中，羊绒周期性的阶段变化已经得到了很好的阐明，但毛发纤维的变化则不太明显。在这里，我们评估了来自同一三头阿尔巴斯绒山羊的 PHF 和 SHF 在羊绒生长期的退行期和休止期的转录组。我们总共在休止期和退行期的 SHF 的 PHF 之间发现了 1977 个差异表达基因（DEGs），在休止期和退行期的 SHF 之间发现了 1199 个 DEGs，在退行期的 SHF 的 PHF 和退行期的 SHF 之间发现了 2629 个 DEGs，在休止期的 SHF 的 PHF 和休止期的 SHF 之间发现了 755 个 DEGs。通过基于 GO 和 KEGG 数据库分析基因功能，我们发现 DEGs 在退行期和休止期的 SHF 之间具有肌肉收缩和肌丝滑动的功能，表明立毛肌可能在退行期到休止期的转变中发挥作用。此外，考虑到 PHF 和 SHF 之间 DEGs 的富集 GO 和 KEGG 类别，我们认为当 SHF 处于退行期时，部分 PHF 可能处于自身的生长期，但当 SHF 处于休止期时，PHF 可能进入休止期。最后，我们强烈推荐了几个可能作为 SHF 从退行期到休止期以及 PHF 在 SHF 的退行期和休止期之间生长阶段转变的调节剂的潜在基因，包括 IL17RB 和 ZNF 的 8 个成员。这些结果为内蒙古绒山羊 SHF 从退行期到休止期的转变以及 PHF 在 SHF 退行期和休止期的生长情况提供了分子机制的深入了解。
