Wang X, Xu H R, Li T, Qu L, Zhao Z D, Zhang Z Y
College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Mol Biol Rep. 2014 Sep;41(9):5665-8. doi: 10.1007/s11033-014-3435-8. Epub 2014 Jun 8.
Keratin-associated protein 9.2 (KAP9.2) and Homeobox C13 (Hoxc13) genes were chosen to study because of their biological functions involving hair formation. KAP9.2 gene belongs to the ultra high sulfur KAPs, which is important for hair formation and may have association with cashmere. Hoxc13 takes part in the formation of cashmere keratin and maintaining the normal structure of follicle. It has been reported that Hoxc13 gene exists binding site of KP and KAP genes at its promoter regions in mouse. So the expression of KAP9.2 and Hoxc13 genes was detected at anagen stage vs telogen stage by qRT-PCR. The data showed that KAP9.2 and Hoxc13 gene had similar expression trend at different stages, which indicated that there was interaction between them. KAP9.2 and Hoxc13 gene had lower expression level in anagen than that of in telogen of cashmere growth. In anagen, KAP9.2 and Hoxc13 expressed lower in high cashmere yield individuals than that of in low cashmere yield ones. In telogen, the result was reverse. The study would provide the evidence of involvement of KAP9.2 and Hoxc13 in hair periodic growth.
由于角蛋白相关蛋白9.2（KAP9.2）和同源盒C13（Hoxc13）基因的生物学功能涉及毛发形成，因此选择对其进行研究。KAP9.2基因属于超高硫角蛋白相关蛋白，对毛发形成很重要，可能与羊绒有关。Hoxc13参与羊绒角蛋白的形成并维持毛囊的正常结构。据报道，在小鼠中，Hoxc13基因在其启动子区域存在角蛋白相关蛋白（KP）和KAP基因的结合位点。因此，通过qRT-PCR检测了KAP9.2和Hoxc13基因在生长期与休止期的表达情况。数据显示，KAP9.2和Hoxc13基因在不同阶段具有相似的表达趋势，这表明它们之间存在相互作用。在羊绒生长的生长期，KAP9.2和Hoxc13基因的表达水平低于休止期。在生长期，高羊绒产量个体中KAP9.2和Hoxc13的表达低于低羊绒产量个体。在休止期，结果相反。该研究将为KAP9.2和Hoxc13参与毛发周期性生长提供证据。
