Bai Chen-Long, Zhao Guang-Rong
Key Laboratory of Systems Bioengineering, Ministry of Education; Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, China.
J Sep Sci. 2015 Aug;38(16):2833-40. doi: 10.1002/jssc.201500416. Epub 2015 Jul 17.
Salvianic acid A (also known as danshensu) is a plant-derived polyphenolic acid, and has a variety of physiological and pharmacological activities. Our laboratory previously constructed an unprecedented artificial biosynthetic pathway in Escherichia coli and established the fermentation process to produce salvianic acid A. Here, we developed an efficient method for separating salvianic acid A from the fermentation broth of engineered Escherichia coli by macroporous resins. Among ten tested macroporous resins, the static and dynamic adsorption/desorption experiments demonstrated that X5 resin was the best to separate salvianic acid A from fermentation broth. Other parameters during static and dynamic procedures were also investigated. Under the optimum separation conditions, the average adsorption capacity of SAA were 10.66±0.54 mg/g dry resin and the desorption ratio was 85.6±4.1%. The purity and recovery yield of salvianic acid A in the final dry product were 90.2±1.5 and 81.5±2.3%, respectively. The results show that adsorption separation with macroporous resin X5 was an efficient method to prepare salvianic acid A from fermentation broth. This work will benefit the development and application of plant-derived salvianic acid A and its derivatives.
丹酚酸A（又称丹参素）是一种植物来源的多酚酸，具有多种生理和药理活性。我们实验室之前在大肠杆菌中构建了一条前所未有的人工生物合成途径，并建立了生产丹酚酸A的发酵工艺。在此，我们开发了一种利用大孔树脂从工程化大肠杆菌发酵液中分离丹酚酸A的高效方法。在十种测试的大孔树脂中，静态和动态吸附/解吸实验表明，X5树脂是从发酵液中分离丹酚酸A的最佳树脂。还研究了静态和动态过程中的其他参数。在最佳分离条件下，丹酚酸A的平均吸附容量为10.66±0.54 mg/g干树脂，解吸率为85.6±4.1%。最终干燥产物中丹酚酸A的纯度和回收率分别为90.2±1.5和81.5±2.3%。结果表明，用大孔树脂X5进行吸附分离是从发酵液中制备丹酚酸A的有效方法。这项工作将有利于植物来源的丹酚酸A及其衍生物的开发和应用。
