Soliman Amel M, Fahmy Sohair R, El-Abied Salma A
Zoology Department, Faculty of Science, Cairo University Egypt.
Faculty of Science, El-Markb University El Khoms, Libya.
Int J Clin Exp Pathol. 2015 Apr 1;8(4):3543-55. eCollection 2015.
With the development of sophisticated instruments for the isolation and elucidation of natural products structures from marine and freshwater organisms, major advances have been made in the discovery of aquatic derived therapeutics. Present investigations were carried out to evaluate cuttlefish (Sepia officinalis) ink extract (IE) and freshwater clam (Coelatura aegyptiaca) extract (CE) for their anticancer and antioxidant activities as compared to 5-flurouracil (5-Fu), in Ehrlich ascites carcinoma (EAC).
Sixty female Swiss albino mice were divided into five groups (n = 12). All groups except group I received EAC cells (5 × 10(6) cells/mouse i.p.) and this was taken as the 0th day. Group I served as saline control (5 ml/kg 0.9% NaCl w/v p.o). Group II served as EAC control. Rats of groups III, IV and V received IE, CE (200 mg/kg body weight i.p.), and reference drug (5-Fu, 20 mg/kg body weight i.p.), respectively.
The reduction in tumor volume, packed cell volume, tumor cell counts and increase in median survival time and percentage increase in life span in treated animals were observed. There was a significant increase in RBC count; Hb content in treated animals and reduction in total WBC count. There was a significant decrease in AST, ALT, ALP and liver MDA levels and increase in GSH, SOD and NO levels were observed in all treated animals.
Both IE and CE were effective in inhibiting the tumor growth in ascitic tumor models. The biochemical, antioxidants and histopathological studies were also supported their antitumor properties.
随着用于从海洋和淡水生物中分离和阐明天然产物结构的精密仪器的发展，在水生来源治疗药物的发现方面取得了重大进展。本研究旨在评估乌贼（Sepia officinalis）墨汁提取物（IE）和淡水蛤（Coelatura aegyptiaca）提取物（CE）与5-氟尿嘧啶（5-Fu）相比，对艾氏腹水癌（EAC）的抗癌和抗氧化活性。
将60只雌性瑞士白化小鼠分为五组（n = 12）。除第一组外，所有组均接种EAC细胞（5×10⁶个细胞/小鼠，腹腔注射），这一天作为第0天。第一组作为生理盐水对照组（5 ml/kg 0.9% NaCl，口服）。第二组作为EAC对照组。第三、四、五组的大鼠分别接受IE、CE（200 mg/kg体重，腹腔注射）和参比药物（5-Fu，20 mg/kg体重，腹腔注射）。
观察到治疗动物的肿瘤体积减小、红细胞压积降低、肿瘤细胞计数减少，中位生存时间延长和寿命延长百分比增加。治疗动物的红细胞计数显著增加；血红蛋白含量增加，白细胞总数减少。所有治疗动物的天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶和肝脏丙二醛水平显著降低，谷胱甘肽、超氧化物歧化酶和一氧化氮水平升高。
IE和CE在腹水肿瘤模型中均能有效抑制肿瘤生长。生化、抗氧化和组织病理学研究也支持了它们的抗肿瘤特性。
