Kang Shirong, Ma Ying, Liu Caixia, Cao Chunli, Qi Jiafeng, Li Jiwei, Wu Xu
Department of Thoracic Surgery, Nanfang Hospital, Southern Medical University Guangzhou 510515, China ; Department of Thoracic Surgery, Affiliated Hospital of Inner Mongolia Medical College Hohhot 010050, China.
Department of Thoracic Surgery, Affiliated Hospital of Inner Mongolia Medical College Hohhot 010050, China.
Int J Clin Exp Pathol. 2015 Apr 1;8(4):4171-6. eCollection 2015.
DNA repair genes is a key factor for cancer susceptibility, and we conducted a case-control study to investigate the association of XRCC1 codons 194 (Arg to Trp), 280 (Arg to His) and 399 (Arg to Gln) with risk of NSCLC. 210 NSCLC patients and 210 health control subjects were randomly selected from Huaihe Hospital between January 2012 and June 2014. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was taken to assess the genotyping of XRCC1 Arg194Trp, Arg280His and Arg399Gln. By multivariate logistic regression analysis, we found individuals carrying with Trp/Trp and Arg/Trp + Trp/Trp genotypes were associated with a significantly increased risk of NSCLC compared with Arg/Arg genotype, and the OR (95% CI) were 3.15 (1.32-8.09) and 1.52 (1.02-2.28), respectively. The potential association of Arg/Trp+ Trp/Trp genotype of XRCC1 Arg194Trp with the risk of NSCLC is more evidence in smokers, and the OR (95% CI) was 1.78 (1.01-3.24). In conclusion, we found that XRCC1 Arg194Trp polymorphism may be associated with NSCLC risk, especially in smokers.
DNA修复基因是癌症易感性的关键因素，我们进行了一项病例对照研究，以调查XRCC1基因密码子194（从精氨酸到色氨酸）、280（从精氨酸到组氨酸）和399（从精氨酸到谷氨酰胺）与非小细胞肺癌风险的关联。2012年1月至2014年6月期间，从淮河医院随机选取了210例非小细胞肺癌患者和210名健康对照者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法评估XRCC1基因Arg194Trp、Arg280His和Arg399Gln的基因分型。通过多因素逻辑回归分析，我们发现携带Trp/Trp和Arg/Trp + Trp/Trp基因型的个体与Arg/Arg基因型相比，患非小细胞肺癌的风险显著增加，其比值比（95%可信区间）分别为3.15（1.32 - 8.09）和1.52（1.02 - 2.28）。XRCC1基因Arg194Trp的Arg/Trp+ Trp/Trp基因型与非小细胞肺癌风险的潜在关联在吸烟者中更明显，比值比（95%可信区间）为1.78（1.01 - 3.24）。总之，我们发现XRCC1基因Arg194Trp多态性可能与非小细胞肺癌风险相关，尤其是在吸烟者中。
