中国云南省蚊媒传播的曼萨尼拉病毒的分离与全基因组分析。

Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China.

作者信息

Feng Yun, Fu Shi-Hong, Yang Wei-Hong, Zhang Yu-Zhen, He Biao, Tu Chang-Chun, Liang Guo-Dong, Zhang Hai-Lin

机构信息

Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, 671000, People's Republic of China.

State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People's Republic of China.

出版信息

BMC Res Notes. 2015 Jun 23;8:255. doi: 10.1186/s13104-015-1198-5.

DOI:10.1186/s13104-015-1198-5

PMID:26100251

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4477421/

Abstract

BACKGROUND

There have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People's Republic of China.

RESULTS

The DHL10M107 L, M and S genes were sequenced at the nucleotide and deduced amino acid levels. The L, M and S gene sequences of DHL10M107 clustered with the MANV strains VN04-2108, TRVL3587, SA An 4165, and AV 782. DHL10M107 was most closely related to VN04-2108. Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108. In terms of amino acid homology, all of the amino acid differences were in the L (96.3% homologous) and M (97.7% homologous) fragments.

CONCLUSIONS

DHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province. This is the first reported isolation of MANV in mainland China.

摘要

背景

迄今为止已鉴定出四种曼萨尼拉病毒（MANV）毒株。在此，我们鉴定了一种从中华人民共和国云南省德宏州瑞丽市的三带喙库蚊中分离出的新型MANV毒株（DHL10M107）。

结果

对DHL10M107的L、M和S基因进行了核苷酸和推导氨基酸水平的测序。DHL10M107的L、M和S基因序列与MANV毒株VN04 - 2108、TRVL3587、SA An 4165和AV 782聚类。DHL10M107与VN04 - 2108关系最为密切。DHL10M107与VN04 - 2108之间的核苷酸同源性在96%至99%之间。在氨基酸同源性方面，所有氨基酸差异都在L片段（同源性96.3%）和M片段（同源性97.7%）中。