Xu Ziqian, Cao Lei, Cai Liang, Fu Shihong, Nie Kai, Yin Qikai, Cao Yuxi, Liu Guoping, Liu Yunzhi, Zhang Hong, Gao Lidong, He Ying, Wang Huanyu, Liang Guodong
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Hunan Center for Disease Control and Prevention, Changsha 410005, China.
Pathogens. 2022 Sep 15;11(9):1049. doi: 10.3390/pathogens11091049.
The Cat Que orthobunyavirus has been found in mosquitoes, birds, pigs, and humans, suggesting its wide range of hosts and potential public health implications. During arbovirus surveillance in 2013, the HN1304M virus was isolated from naturally occurring biting midges in Hunan Province, southern China. The virus was cytopathic to BHK-21 cells and showed stable passage, but was not cytopathic to C6/36 cells. Determination and analysis of the viral genome sequence revealed that HN1304M is an RNA virus with three gene segments, namely, L, M, and S. The nucleotide and amino acid sequence homologies of HN1304M to Cat Que viruses in the Manzanilla species complex were 90.3-99.4%, and 95-100%, respectively, while the homologies to other viruses in this species complex were 74-86.6% and 78.1-96.1%, respectively. A phylogenetic analysis of the viral genes revealed that HN1304M formed an evolutionary branch with other Cat Que viruses isolated from mosquitoes, pigs, birds, and humans, which was completely independent of the other viruses in this complex. The fact that the Cat Que virus was isolated from suggests that biting midges may participate in the natural circulation of Cat Que viruses.
已在蚊子、鸟类、猪和人类中发现了猫鹊正布尼亚病毒，这表明其宿主范围广泛且对公共卫生具有潜在影响。在2013年虫媒病毒监测期间，从中国南方湖南省自然出现的蠓中分离出HN1304M病毒。该病毒对BHK-21细胞具有细胞病变作用且传代稳定，但对C6/36细胞无细胞病变作用。病毒基因组序列的测定和分析表明，HN1304M是一种具有L、M和S三个基因片段的RNA病毒。HN1304M与曼萨尼利亚种复合群中猫鹊病毒的核苷酸和氨基酸序列同源性分别为90.3%-99.4%和95%-100%，而与该种复合群中其他病毒的同源性分别为74%-86.6%和78.1%-96.1%。对病毒基因的系统发育分析表明，HN1304M与从蚊子、猪、鸟类和人类中分离出的其他猫鹊病毒形成了一个进化分支，该分支完全独立于该复合群中的其他病毒。从蠓中分离出猫鹊病毒这一事实表明，蠓可能参与了猫鹊病毒的自然循环。
