Feng Yun, Zhang Yuzhen, Yang Weihong, Zhang Hailin
Bing Du Xue Bao. 2016 Mar;32(2):161-9.
We wished to sequence the full-length genomes of the DHL10M110 strain of the Akabane virus (AKV) isolated from mosquitoes in Yunnan Province, China, in 2010. We also wished to analyze the characteristics of these complete nucleotide sequences. The complete genomic sequence of the DHL10M110 strain from Yunnan Province was obtained by reverse transcription-polymerase chain reaction and direct sequencing. We found that the length of the L, M and S gene nucleotide sequences of the DHL10M110 strain were 6 869-bp, 4 309-bp and 856-bp, respectively, including the open reading frame (ORF) nucleotide sequences of 6 756-bp (L), 4 206-bp (M) and 702-bp (S), encoding 2252, 1402 and 234 amino-acid polyproteins, respectively. Phylogenetic analyses based on L-fragment ORF showed that the DHL10M110 strain had a close relationship with the OBE-1 strain of the AKV from Japan and AKVS-7/SKR/2010 strain of the AKV from South Korea. Phylogenetic analyses based on M- and S-fragment ORF showed that the DHL10M110 strain had a close relationship with the epidemic strains of the AKV from Japan, South Korea and Taiwan, but that the DHL10M110 strain had a lone evolutionary branch. In terms of nucleotide (amino acid) homology, the similarity of L-, M- and S-fragment ORFs of the DHL10M110 strain to the OBE-1 strain from Japan was 92.6% (98%), 88.5% (94%) and 96.4% (99.1%), respectively. When comparing the DHL10M110 strain with the OBE-1 strain, we noted 45, 84, and 2 different sites in the amino acids of L, M and S fragments, respectively. Homology and phylogenetic analyses also suggested that the DHL10M110 strain had a distant relationship with the epidemic strains of the AKV from Kenya and Australia. Also, we confirmed by complete genomic sequence analyses that the DHL10M110 strain was clade-Asia of the AKV. However, differences between the DHL10M110 strain compared with strains from Japan and South Korea were also noted. These results suggest that the DHL10M110 strain harbored relatively stable genetic characteristics and distinct regional features. This is the first time that full-length genomic sequences of the DHL10M110 strain of the AKV in mainland China have been obtained.
我们希望对2010年从中国云南省蚊子中分离出的赤羽病毒（AKV）DHL10M110株的全长基因组进行测序。我们还希望分析这些完整核苷酸序列的特征。通过逆转录-聚合酶链反应和直接测序获得了来自云南省的DHL10M110株的完整基因组序列。我们发现，DHL10M110株的L、M和S基因核苷酸序列长度分别为6869bp、4309bp和856bp，包括开放阅读框（ORF）核苷酸序列6756bp（L）、4206bp（M）和702bp（S），分别编码2252、1402和234个氨基酸的多聚蛋白。基于L片段ORF的系统发育分析表明，DHL10M110株与来自日本的AKV的OBE-1株和来自韩国的AKV的AKVS-7/SKR/2010株关系密切。基于M和S片段ORF的系统发育分析表明，DHL10M110株与来自日本、韩国和台湾的AKV流行株关系密切，但DHL10M110株有一个单独的进化分支。在核苷酸（氨基酸）同源性方面，DHL10M110株的L、M和S片段ORF与来自日本的OBE-1株的相似性分别为92.6%（98%）、88.5%（94%）和96.4%（99.1%）。在将DHL10M110株与OBE-1株进行比较时，我们分别在L、M和S片段的氨基酸中发现了45、84和2个不同位点。同源性和系统发育分析还表明，DHL10M110株与来自肯尼亚和澳大利亚的AKV流行株关系较远。此外，我们通过完整基因组序列分析证实，DHL10M110株是AKV的亚洲分支。然而，也注意到DHL10M110株与来自日本和韩国的毒株之间的差异。这些结果表明，DHL10M110株具有相对稳定的遗传特征和独特的区域特征。这是首次在中国大陆获得AKV的DHL10M110株的全长基因组序列。
