利用人杂交瘤技术分离人单克隆抗体。
Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies.
机构信息
Vanderbilt Vaccine Center and Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232.
Vanderbilt Vaccine Center and Departments of Pediatrics and Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232.
出版信息
Microbiol Spectr. 2015 Feb;3(1):AID-0027-2014. doi: 10.1128/microbiolspec.AID-0027-2014.
Abstract
The human hybridoma technique offers an important approach for isolation of human monoclonal antibodies. A diversity of approaches can be used with varying success. Recent technical advances in expanding the starting number of human antigen-specific B cells, improving fusion efficiency, and isolating new myeloma partners and new cell cloning methods have enabled the development of protocols that make the isolation of human monoclonal antibodies from blood samples feasible. Undoubtedly, additional innovations that could improve efficiency are possible.
摘要
人杂交瘤技术为人单克隆抗体的分离提供了一种重要的方法。可以使用多种方法，但其成功率各不相同。最近在扩大人抗原特异性 B 细胞起始数量、提高融合效率、分离新的骨髓瘤伙伴和新的细胞克隆方法方面的技术进步，使从血液样本中分离人单克隆抗体的方案成为可能。毫无疑问，还可能有其他提高效率的创新。
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本文引用的文献
1
Human monoclonal antibodies derived from a patient infected with 2009 pandemic influenza A virus broadly cross-neutralize group 1 influenza viruses.
Biochem Biophys Res Commun. 2014 Jul 18;450(1):42-8. doi: 10.1016/j.bbrc.2014.05.060. Epub 2014 May 22.
2
Production of monoclonal antibodies.
Curr Protoc Immunol. 2013 Oct 1;102:2.5.1-2.5.29. doi: 10.1002/0471142735.im0205s102.
3
A method for high-frequency intergeneric fusion of plant protoplasts.
Planta. 1974 Dec;115(4):355-67. doi: 10.1007/BF00388618.
4
Variables influencing anti-human immunodeficiency virus type 1 neutralizing human monoclonal antibody (NhMAb) production among infected Thais.
Southeast Asian J Trop Med Public Health. 2013 Sep;44(5):825-41.
5
The potent and broadly neutralizing human dengue virus-specific monoclonal antibody 1C19 reveals a unique cross-reactive epitope on the bc loop of domain II of the envelope protein.
mBio. 2013 Nov 19;4(6):e00873-13. doi: 10.1128/mBio.00873-13.
6
Human monoclonal antibodies derived from memory B cells following live attenuated dengue virus vaccination or natural infection exhibit similar characteristics.
J Infect Dis. 2013 Jun 15;207(12):1898-908. doi: 10.1093/infdis/jit119. Epub 2013 Mar 22.
7
IL-21 and CD40L synergistically promote plasma cell differentiation through upregulation of Blimp-1 in human B cells.
J Immunol. 2013 Feb 15;190(4):1827-36. doi: 10.4049/jimmunol.1201678. Epub 2013 Jan 16.
8
Persistence of circulating memory B cell clones with potential for dengue virus disease enhancement for decades following infection.
J Virol. 2012 Mar;86(5):2665-75. doi: 10.1128/JVI.06335-11. Epub 2011 Dec 14.
9
An ATM/Chk2-mediated DNA damage-responsive signaling pathway suppresses Epstein-Barr virus transformation of primary human B cells.
Cell Host Microbe. 2010 Dec 16;8(6):510-22. doi: 10.1016/j.chom.2010.11.004.
10
Human monoclonal antibodies to West Nile virus identify epitopes on the prM protein.
Virology. 2011 Feb 5;410(1):30-7. doi: 10.1016/j.virol.2010.10.033. Epub 2010 Nov 16.
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