利用人杂交瘤技术分离人单克隆抗体。
Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies.
机构信息
Vanderbilt Vaccine Center and Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232.
Vanderbilt Vaccine Center and Departments of Pediatrics and Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232.
出版信息
Microbiol Spectr. 2015 Feb;3(1):AID-0027-2014. doi: 10.1128/microbiolspec.AID-0027-2014.
Abstract
The human hybridoma technique offers an important approach for isolation of human monoclonal antibodies. A diversity of approaches can be used with varying success. Recent technical advances in expanding the starting number of human antigen-specific B cells, improving fusion efficiency, and isolating new myeloma partners and new cell cloning methods have enabled the development of protocols that make the isolation of human monoclonal antibodies from blood samples feasible. Undoubtedly, additional innovations that could improve efficiency are possible.
摘要
人杂交瘤技术为人单克隆抗体的分离提供了一种重要的方法。可以使用多种方法，但其成功率各不相同。最近在扩大人抗原特异性 B 细胞起始数量、提高融合效率、分离新的骨髓瘤伙伴和新的细胞克隆方法方面的技术进步，使从血液样本中分离人单克隆抗体的方案成为可能。毫无疑问，还可能有其他提高效率的创新。
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