Algarni Amnah Abdullah, Lippert Frank, Hara Anderson Takeo
Department of Preventive and Community Dentistry, School of Dentistry, Indiana University, Indianapolis, IN, USA.
Braz Oral Res. 2015;29. doi: 10.1590/1807-3107BOR-2015.vol29.0081.
The aim of this study was to compare the protective effects of solutions containing stannous (Sn), fluoride (F) and their combination in the prevention of dentin erosion. Forty bovine root dentin specimens (4'4'2 mm(3)) were prepared and randomly assigned to 4 groups (n = 10): SnCl2 (800 ppm/6.7 mM Sn), NaF (250 ppm/13 mM F), NaF/SnCl2 (800 ppm/6.7 mM Sn; 250 ppm/13 mM F), and deionized water (DIW) as a negative control. An acquired pellicle was formed on dentin samples by incubation in clarified, pooled, stimulated human saliva for 24 hours. The specimens were subjected to 5 daily cycles, each consisting of 5 of min demineralization (0.3%/15.6 mM citric acid, pH 2.6, 6'/day) and 60 min of re-mineralization in clarified human saliva. Thirty minutes after the 1st, 3rd and 5th demineralization episodes of each day, the specimens were treated with one of the test solutions for 2 min. Surface loss was measured via optical profilometry. Mixed-model ANOVA followed by Tukey's test were used for the statistical analysis. Sn, F, and their combination significantly reduced the dentin surface loss by 23%, 36%, and 60% compared with DIW, respectively. All groups were significantly different (p < 0.05). The combination of Sn and F significantly reduced the amount of dentin surface loss compared with all other groups. The F group also significantly reduced surface loss compared with Sn and DIW, followed by the Sn group, which showed significantly greater protection compared with the DIW control. The daily use of a combined fluoride and stannous solution is promising for preventing dentin erosion.
本研究的目的是比较含锡（Sn）、氟（F）及其组合的溶液在预防牙本质侵蚀方面的保护作用。制备了40个牛牙根牙本质标本（4×4×2 mm³），并随机分为4组（n = 10）：SnCl₂（800 ppm/6.7 mM Sn）、NaF（250 ppm/13 mM F）、NaF/SnCl₂（800 ppm/6.7 mM Sn；250 ppm/13 mM F），以及作为阴性对照的去离子水（DIW）。通过在澄清的、汇集的、刺激的人类唾液中孵育24小时，在牙本质样本上形成获得性薄膜。标本每天进行5个循环，每个循环包括5分钟的脱矿质处理（0.3%/15.6 mM柠檬酸，pH 2.6，每天6次）和60分钟在澄清人类唾液中的再矿化处理。在每天第1次、第3次和第5次脱矿质处理后30分钟，用其中一种测试溶液处理标本2分钟。通过光学轮廓测量法测量表面损失。采用混合模型方差分析，随后进行Tukey检验进行统计分析。与DIW相比，Sn、F及其组合分别使牙本质表面损失显著降低了23%、36%和60%。所有组之间均有显著差异（p < 0.05）。与所有其他组相比，Sn和F的组合显著减少了牙本质表面损失量。F组与Sn组和DIW组相比也显著减少了表面损失，其次是Sn组，其显示出比DIW对照组显著更大的保护作用。每日使用氟化物和亚锡联合溶液在预防牙本质侵蚀方面具有前景。
