Balfanz J, Rautenberg P
Dept. of Medical Microbiology, University of Kiel, FRG.
Biochem Biophys Res Commun. 1989 Dec 29;165(3):1364-70. doi: 10.1016/0006-291x(89)92754-x.
Toxin A (enterotoxin) and toxin B (cytotoxin) of Clostridium difficile were both inactivated by the arginine specific reagent 1,2-cyclohexanedione. Molecular stability during the inactivation process was demonstrated by SDS-PAGE analysis showing the same migration rates for modified and unmodified forms of the 230 kDa toxin A and of the 250 kDa toxin B. Cytotoxicity of both toxins as well as mouse lethality of the enterotoxin were drastically decreased as a result of the arginine modification. The reaction followed pseudo-first-order kinetics. Analysis of the data suggested that modification of a single arginine residue was sufficient to abolish the activity of both toxins.
艰难梭菌的毒素A（肠毒素）和毒素B（细胞毒素）均被精氨酸特异性试剂1,2 - 环己二酮灭活。SDS - PAGE分析表明，在灭活过程中分子稳定性得以体现，其显示230 kDa的毒素A和250 kDa的毒素B的修饰形式和未修饰形式具有相同的迁移率。精氨酸修饰导致两种毒素的细胞毒性以及肠毒素的小鼠致死率大幅降低。该反应遵循假一级动力学。数据分析表明，单个精氨酸残基的修饰足以消除两种毒素的活性。
