Rautenberg P, Stender F, Ullmann U
Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Feb;261(1):29-42. doi: 10.1016/s0176-6724(86)80060-8.
A clinical isolate of Clostridium difficile has been tested for its toxin production. Both toxins, toxin A and toxin B, could be detected by tissue culture and in animal models as well. Antibodies against a crude toxin A preparation have been prepared. These antibodies are able to neutralize the toxin both in the mouse lethality test and tissue culture test systems. The specificity of this antiserum has been analysed by electroimmunoprecipitation methods. Using immunoblotting, it could be demonstrated that the antigenicity of toxin A after SDS polyacrylamide gel electrophoresis under denaturing and reducing conditions was still preserved. The molecular weight of toxin A has been estimated to be 250000. Immunoblotting offers a simple and reliable procedure for toxin A detection from culture supernatants of C. difficile.
已对艰难梭菌的一株临床分离株进行毒素产生检测。毒素A和毒素B这两种毒素均可通过组织培养以及在动物模型中检测到。已制备了针对粗制毒素A制剂的抗体。这些抗体在小鼠致死性试验和组织培养试验系统中均能中和毒素。已通过电免疫沉淀法分析了该抗血清的特异性。使用免疫印迹法可以证明，在变性和还原条件下进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，毒素A的抗原性仍然保留。毒素A的分子量估计为250000。免疫印迹法为从艰难梭菌培养上清液中检测毒素A提供了一种简单可靠的方法。
