用于评估海洋藻类提取物抗菌活性的纸片扩散法。

Disk Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts.

作者信息

Desbois Andrew P, Smith Valerie J

机构信息

Marine Biotechnology Research Group, Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirlingshire, FK9 4LA, United Kingdom,

出版信息

Methods Mol Biol. 2015;1308:403-10. doi: 10.1007/978-1-4939-2684-8_25.

DOI:10.1007/978-1-4939-2684-8_25

PMID:26108520

Abstract

Marine algae are a relatively untapped source of bioactive natural products, including those with antimicrobial activities. The ability to assess the antimicrobial activity of cell extracts derived from algal cultures is vital to identifying species that may produce useful novel antibiotics. One assay that is used widely for this purpose is the disk diffusion assay due to its simplicity, rapidity, and low cost. Moreover, this assay gives output data that are easy to interpret and can be used to screen many samples at once irrespective of the solvent used during preparation. In this chapter, a step-by-step protocol for performing a disk diffusion assay is described. The assay is particularly well suited to testing algal cell extracts and fractions resulting from separation through bioassay-guided approaches.

摘要

海洋藻类是生物活性天然产物的一个相对未被开发的来源，包括那些具有抗菌活性的产物。评估从藻类培养物中获得的细胞提取物的抗菌活性，对于识别可能产生有用新型抗生素的物种至关重要。由于其简单、快速和低成本，圆盘扩散法是广泛用于此目的的一种检测方法。此外，该检测方法给出的输出数据易于解释，并且无论在制备过程中使用何种溶剂，都可用于一次性筛选许多样品。在本章中，描述了进行圆盘扩散法检测的分步方案。该检测方法特别适合于测试藻类细胞提取物以及通过生物测定引导方法分离得到的组分。