Han Jianying, Zhang Jingyu, He Wenni, Huang Pei, Oyeleye Ayokunmi, Liu Xueting, Zhang Lixin
Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Methods Mol Biol. 2015;1263:187-96. doi: 10.1007/978-1-4939-2269-7_15.
Traditional Chinese medicines (TCMs) serve as a major source of a variety of drug lead compounds. In the process of natural products development, bioassay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purification of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liquid chromatography (HPLC) are described in detail.
中药是多种药物先导化合物的主要来源。在天然产物开发过程中，生物活性测定导向分离是一种快速且经过验证的分离具有生物活性化合物的方法。本章介绍了中药粗提物中化合物的生物活性测定导向分离与纯化。详细描述了包括尺寸排阻色谱法（SEC）和高效液相色谱法（HPLC）在内的两种方法。
