三丁酸甘油酯补充可改善宫内生长受限仔猪的生长及肠道消化和屏障功能。
Supplementation of tributyrin improves the growth and intestinal digestive and barrier functions in intrauterine growth-restricted piglets.
机构信息
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
出版信息
Clin Nutr. 2016 Apr;35(2):399-407. doi: 10.1016/j.clnu.2015.03.002. Epub 2015 Mar 13.
BACKGROUND & AIMS: Intrauterine growth restriction (IUGR) neonates suffer from growth restriction. Tributyrin (TB), a pro-drug of butyrate, can facilitate the growth of animals. This study was to investigate the effects of TB supplementation on the growth of IUGR neonatal piglets.
METHODS
Sixteen IUGR and 8 NBW (normal body weight) neonatal piglets were chosen, weaned at 7th day and fed basic milk diets (NBW and IUGR group) or the basic diets supplemented with 0.1% tributyrin (IT group, IUGR piglets fed with tributyrin) until day 21 (n = 8). The body weights of the piglets on days 0, 7, 10, 14, 17, and 20 were measured. The digestive enzyme activity, intestinal morphology, immunoglobulin levels and gene expression of IgG, FcRn and GPR41 in the small intestines were analyzed.
RESULTS
The body weights of the piglets in the IUGR and IT group were similar, and both were lower than the NBW group on days 10 and 14. However, after day 17, the IT group exhibited improved (P < 0.05) body weights compared to that of the IUGR group. The piglets were sacrificed on day 21. Compared with the NBW piglets, IUGR impaired the development of immune organs and small intestines, impaired the intestinal villus morphology, decreased (P < 0.05) most of the tested intestinal digestive enzyme activities, decreased (P < 0.05) the ileal sIgA and IgG levels, and down-regulated (P < 0.05) the intestinal IgG and GPR41 expression. Piglets in the IT group exhibited a better-developed (P < 0.05) spleen and small intestines, improved intestinal villus morphology, increased (P < 0.05) intestinal villus surface areas, enhanced (P < 0.05) digestive enzyme activities, and up-regulated (P < 0.05) expression of IgG and GPR41 mRNA compared to those of the IUGR group.
CONCLUSIONS
TB supplementation improves the growth and the intestinal digestive and barrier functions in IUGR piglets during the suckling period.
背景与目的
宫内生长受限（IUGR）新生儿存在生长受限的问题。丁酸盐前体三丁酸甘油酯（TB）可促进动物生长。本研究旨在探讨补充 TB 对 IUGR 新生仔猪生长的影响。
方法
选择 16 头 IUGR 和 8 头 NBW（正常体重）新生仔猪，于第 7 天断奶，分别给予基础牛奶日粮（NBW 和 IUGR 组）或基础日粮中添加 0.1%三丁酸甘油酯（IT 组，IUGR 仔猪用三丁酸甘油酯喂养），直至第 21 天（n=8）。在 0、7、10、14、17 和 20 天测量仔猪体重。分析仔猪的消化酶活性、肠道形态、免疫球蛋白水平以及小肠 IgG、FcRn 和 GPR41 的基因表达。
结果
IUGR 组和 IT 组仔猪体重相似，第 10 和 14 天均低于 NBW 组。但 17 天后，IT 组体重增长优于 IUGR 组（P<0.05）。第 21 天处死仔猪。与 NBW 仔猪相比，IUGR 损伤免疫器官和小肠发育，降低（P<0.05）肠绒毛形态，降低（P<0.05）大部分测试的肠道消化酶活性，降低（P<0.05）回肠 sIgA 和 IgG 水平，并下调（P<0.05）肠道 IgG 和 GPR41 表达。IT 组仔猪表现出更好发育的（P<0.05）脾脏和小肠，改善肠绒毛形态，增加（P<0.05）肠绒毛表面积，增强（P<0.05）消化酶活性，上调（P<0.05）IgG 和 GPR41 mRNA 的表达。
结论
在哺乳期，补充 TB 可改善 IUGR 仔猪的生长和肠道消化及屏障功能。
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