Li Runlin, Ding Xuedong, Lei Mingkang, Li Panpan, Giannenas Ilias, Wang Jing, Zhu Weiyun
National Center for International Research on Animal Gut Nutrition, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Aristotle University of Thessaloniki, University Campus, Thessaloniki 54124, Greece.
Anim Nutr. 2024 Dec 3;20:131-144. doi: 10.1016/j.aninu.2024.11.008. eCollection 2025 Mar.
It has been found that thymol (Thy) and rosmarinic acid (Ros-A) improve the growth performance of piglets and relieve intestinal inflammation in animals. The effects of Thy and Ros-A separately or in combination (Thy × Ros-A) on the intestinal function and health of piglets challenged with K88 ( K88) were investigated. A total of 30 piglets aged 21 d were assigned to 5 groups ( = 6). The control (Con) and K88 groups piglets received a basal diet, while the Thy, Ros-A, and Thy × Ros-A groups were fed a basal diet supplemented with 500 mg/kg Thy, 500 mg/kg Ros-A, and 250 mg/kg Thy + 250 mg/kg Ros-A, respectively. On the 19th and 20th day, piglets in the K88, Thy, Ros-A, and Thy × Ros-A groups were orally administered 10 mL of phosphate-buffered saline (PBS) containing approximately 1 × 10 CFU/mL of K88, while the Con group received an equal volume of PBS. The results showed that the Thy × Ros-A treatment reduced the damage to ileal villi induced by the K88 challenge, leading to longer villi in the ileum ( < 0.05). Thy and Ros-A modulated the composition of the ileal microbiota. Compared to the K88 group, the Thy × Ros-A group had a higher abundance of and , while and were lower ( < 0.05). Additionally, the Thy × Ros-A group showed elevated levels of gene and protein expressions for zonula occludens-1, occludin, and claudin-1 compared to the K88 group ( < 0.05). In conclusion, combining Thy and Ros-A reduced ileal damage and relieved the inflammation in weaned piglets challenged with K88 by regulating intestinal microflora and improving barrier function.
已发现百里香酚（Thy）和迷迭香酸（Ros-A）可改善仔猪的生长性能，并减轻动物的肠道炎症。研究了Thy和Ros-A单独或联合使用（Thy×Ros-A）对受K88挑战的仔猪肠道功能和健康的影响。将30头21日龄的仔猪分为5组（每组n = 6）。对照组（Con）和K88组仔猪饲喂基础日粮，而Thy组、Ros-A组和Thy×Ros-A组分别饲喂添加500 mg/kg Thy、500 mg/kg Ros-A和250 mg/kg Thy + 250 mg/kg Ros-A的基础日粮。在第19天和第20天，K88组、Thy组、Ros-A组和Thy×Ros-A组的仔猪口服10 mL含约1×10⁸ CFU/mL K88的磷酸盐缓冲盐水（PBS），而Con组接受等体积的PBS。结果表明，Thy×Ros-A处理减轻了K88挑战诱导的回肠绒毛损伤，使回肠绒毛更长（P < 0.05）。Thy和Ros-A调节了回肠微生物群的组成。与K88组相比，Thy×Ros-A组中[具体菌属1]和[具体菌属2]的丰度较高，而[具体菌属3]和[具体菌属4]较低（P < 0.05）。此外，与K88组相比，Thy×Ros-A组中紧密连接蛋白-1、闭合蛋白和闭锁小带蛋白-1的基因和蛋白表达水平升高（P < 0.05）。总之，Thy和Ros-A联合使用可减轻断奶仔猪受K88挑战后的回肠损伤，并通过调节肠道微生物群和改善屏障功能缓解炎症。
