Zhou Yan-Feng, Duan Jin-Rong, Liu Kai, Xu Dong-Po, Zhang Min-Ying, Fang Di-An, Xu Pao
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Xuejiali 69, 214128, China.
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Xuejiali 69, 214128, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Xuejiali 69, 214128, China.
Mar Genomics. 2015 Dec;24 Pt 3:241-3. doi: 10.1016/j.margen.2015.06.007. Epub 2015 Jun 23.
RNA-Seq technology has been widely applied to transcriptomics, genomics and functional gene study. Here, we performed de novo transcriptome sequencing to produce 23,842,172 clean reads representing a total of 4,815,798,404 (4.8 Gb) nucleotides from comprehensive transcript dataset for testis of Coilia nasus. Over 20 million Illumina reads were assembled into 194,636 unigenes, and 42,642 annotated genes were predicted by Blastx and ESTScan, respectively. Applying Blast analysis and functional annotation (e.g., GO, COG, SwissProt and KEGG) using the assembled gene models from catalogs of other species, we have sampled an extensive and diverse expressed gene catalog for C. nasus representing a large proportion of the genes involved in the onset of spermatogenesis. The results will provide a general clue to the potential spermatogenesis molecular mechanisms for this species.
RNA测序技术已广泛应用于转录组学、基因组学和功能基因研究。在此，我们进行了从头转录组测序，从刀鲚睾丸的综合转录组数据集中获得了23,842,172条clean reads，共代表4,815,798,404（4.8 Gb）个核苷酸。超过2000万条Illumina reads被组装成194,636个单基因，分别通过Blastx和ESTScan预测出42,642个注释基因。利用来自其他物种目录的组装基因模型进行Blast分析和功能注释（如GO、COG、SwissProt和KEGG），我们为刀鲚获取了一个广泛且多样的表达基因目录，该目录涵盖了参与精子发生起始的大部分基因。这些结果将为该物种潜在的精子发生分子机制提供一个总体线索。
