Feng Xiao-Ting, Yang Xue-Jun, Ruan Jian-Jian, Wang Ya-Qi, Zhou Yan-Feng, Xu Dong-Po, Fang Di-An
Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affaris. Freshwater Fisheries Research Center, CAFS, WuXi, 214081, China.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
BMC Dev Biol. 2019 Apr 25;19(1):9. doi: 10.1186/s12861-019-0187-7.
Coilia nasus oogenesis/spawning migration is a well-defined synchronous arrangement process. DnaJs are indispensable molecular chaperones for oogenesis process. However, how DnaJs involved the anadromous spawning migration mechanism is outstanding and plausible.
In this regard, two DnaJs (Cn-DnaJa1 and Cn-DnaJb1) are cloned from the Coilia nasus's ovary. Their structure both contains J domain, G/F domain and ZF domain. Their mRNA transcripts were found extensively expressed in all the sampled tissues and significantly highly in gonads, which probably mean that DnaJs involved in C. nasus's gonad development basal metabolic processes. In the process of spawning migration, Cn-DnaJa1 and Cn-DnaJb1 mRNA transcripts were also expressed with significant differences during oogenesis with highest levels in the development phase, and maintaining high levels during the multiplication, mature and spawning phase. Further study showed that the DnaJa1and DnaJb1protein have high distribution in the onset phase and mainly distributed in the oocyte cytoplasm especially during the migration development phase's.
This experiment study demonstrated that DnaJs participate in reproductive regulation during the spawning migration process in C. nasus and possibly play a vital role in the ovary development process. These findings also provided a base knowledge for further molecular mechanism study of spawning migration.
刀鲚卵子发生/产卵洄游是一个明确的同步排列过程。DnaJ蛋白是卵子发生过程中不可或缺的分子伴侣。然而，DnaJ蛋白如何参与溯河产卵洄游机制尚不清楚且值得探讨。
在这方面，从刀鲚卵巢中克隆出两种DnaJ蛋白（Cn-DnaJa1和Cn-DnaJb1）。它们的结构均包含J结构域、G/F结构域和ZF结构域。发现它们的mRNA转录本在所有采样组织中广泛表达，在性腺中显著高表达，这可能意味着DnaJ蛋白参与刀鲚性腺发育的基础代谢过程。在产卵洄游过程中，Cn-DnaJa1和Cn-DnaJb1的mRNA转录本在卵子发生过程中也有显著差异表达，在发育阶段表达水平最高，在增殖、成熟和产卵阶段保持高水平。进一步研究表明，DnaJa1和DnaJb1蛋白在起始阶段分布较高，主要分布在卵母细胞胞质中，尤其是在洄游发育阶段。
本实验研究表明，DnaJ蛋白参与刀鲚产卵洄游过程中的生殖调控，可能在卵巢发育过程中起重要作用。这些发现也为进一步研究产卵洄游的分子机制提供了基础知识。
