Zheng Xiong, Huang Haining, Su Yinglong, Wei Yuanyuan, Chen Yinguang
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China E-mail:
Water Sci Technol. 2015;72(1):99-105. doi: 10.2166/wst.2015.194.
The pervasive use of engineered nanoparticles (NPs) in a wide range of fields raises concerns about their potential environmental impacts. Previous studies confirmed that some NPs had already entered wastewater treatment plants (WWTPs). Wastewater nutrient removal depends on the metabolisms of activated sludge bacteria and their related key enzymes. Therefore, this study compared the possible influences of Al2O3, SiO2, TiO2, and ZnO NPs on the key enzymes activities and microbial community structures involved in wastewater treatment facilities. It was found that long-term exposure to these NPs significantly affected the microbial communities and changed the relative abundances of key functional bacteria, such as ammonia-oxidizing bacteria. Also, the gene expressions and catalytic activities of essential enzymes, such as ammonia monooxygenase, nitrite oxidoreductase, nitrate reductase, and nitrite reductase, were decreased, which finally resulted in a lower efficiency of biological nitrogen removal.
工程纳米颗粒（NPs）在广泛领域的普遍使用引发了人们对其潜在环境影响的担忧。先前的研究证实，一些纳米颗粒已经进入了污水处理厂（WWTPs）。废水营养物的去除取决于活性污泥细菌的代谢及其相关关键酶。因此，本研究比较了Al2O3、SiO2、TiO2和ZnO纳米颗粒对污水处理设施中关键酶活性和微生物群落结构的可能影响。研究发现，长期暴露于这些纳米颗粒会显著影响微生物群落，并改变关键功能细菌的相对丰度，如氨氧化细菌。此外，氨单加氧酶、亚硝酸氧化还原酶、硝酸还原酶和亚硝酸还原酶等必需酶的基因表达和催化活性均降低，最终导致生物脱氮效率降低。
