State Educational Institution All-Russian Research Institute of Beef Cattle Breeding, 29, 9-Yanvarya Street, Orenburg, Russia, 460000.
Orenburg State University, Pobedy pr. 13, Orenburg, Russia, 460018.
Environ Sci Pollut Res Int. 2018 Jun;25(18):18109-18120. doi: 10.1007/s11356-018-1991-5. Epub 2018 Apr 24.
The research included the study of influence of ultrafine particle preparations (nanoparticles of copper, zinc, iron, CuZn alloy) and metal salts (iron pyrophosphate, copper asparginate, zinc asparginate) on the composition of cecal microbiota of broiler chickens. Before adding the studied nanoparticles and metal salts to the diet, cecal microbiota of broiler chickens was represented by 76% Firmicutes taxon and 16% Bacteroidetes. Numerous among them were the bacteria of the taxa Anaerotruncus spp., Lactobacillus spp., Blautia spp., Alistipes spp., and Bacteroides spp.; they constituted 18, 17, 11, and 6%, respectively. A peculiarity of action of the most analyzed metals in nanoform and in the form of salts was a decrease in the number of phylum Firmicutes bacteria and an increase in the number of microorganisms of the phylum Bacteroidetes. The number of bacteria belonging to the families Ruminococcaceae (III, IV, V, VII, and VIII groups), Bacteroidaceae (in all experimental groups), and Lachnospiraceae (I, IV, V, and VII groups) was registered within the taxa of Firmicutes and Bacteroidetes. At the same time, in some experimental groups, the number of bacteria of the family Lachnospiraceae (II, III, and VIII) decreased in the intestine. The data obtained can be used to assess the possibility of using metal nanoparticles in the poultry diet, as a micronutrient preparation, to correct dysbiosis and to improve the utilization of fodder energy.
该研究包括研究超细微粒制剂（铜、锌、铁的纳米粒子、CuZn 合金）和金属盐（焦磷酸铁、天冬氨酸铜、天冬氨酸锌）对肉鸡盲肠微生物群组成的影响。在将研究用纳米粒子和金属盐添加到饲料中之前，肉鸡盲肠微生物群由 76%的厚壁菌门和 16%的拟杆菌门组成。其中大量存在的细菌包括 Anaerotruncus 属、乳酸杆菌属、布劳特氏菌属、Alistipes 属和拟杆菌属；它们分别占 18%、17%、11%和 6%。最受分析的金属以纳米形式和盐形式作用的一个特点是厚壁菌门细菌数量减少，拟杆菌门微生物数量增加。属于瘤胃球菌科（III、IV、V、VII 和 VIII 组）、拟杆菌科（所有实验组）和lachnospiraceae 科（I、IV、V 和 VII 组）的细菌数量在Firmicutes 和 Bacteroidetes 分类群中均有登记。同时，在一些实验组中，肠道中lachnospiraceae 科（II、III 和 VIII）的细菌数量减少。所得数据可用于评估将金属纳米粒子作为微量营养素制剂用于家禽饲料以纠正肠道菌群失调和提高饲料能量利用率的可能性。
