Fernández Estibalitz, Rodríguez Gelen, Cócera Mercedes, Barbosa-Barros Lucyanna, Alonso Cristina, López-Iglesias Carmen, Jawhari Tariq, de la Maza Alfonso, López Olga
Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Jordi Girona, Barcelona, 18-26, 08034, Spain.
Phys Chem Chem Phys. 2015 Jul 28;17(28):18710-21. doi: 10.1039/c5cp02052b.
Phospholipid-based nanostructures, bicelles and bicosomes, are proposed as carriers of the antioxidant β-carotene. The stability of these nanostructures and their carotenoid cargo was evaluated in an oxidation environment induced by ultraviolet A, visible and infrared A radiation (UVA-VIS-IRA). Additionally, the effect of these nanoaggregates on non-irradiated and irradiated skin microstructure was studied. The characterization of the lipid systems was performed using dynamic light scattering (DLS) and cryo-transmission electron microscopy (Cryo-TEM) and lipid peroxidation of the systems was determined by thiobarbituric acid (TBARS) assay. Moreover, the stability of β-carotene in these lipid systems under this radiation was investigated using Raman spectroscopy. The results showed that the particle size of the bicelles did not change due to radiation. However, the size of the bicosomes increased slightly after irradiation. The TBARS assay showed the absence of peroxides in the bicelles and bicosomes, indicating the preservation of the lipid molecules under the radiation used. Raman experiments showed that bicosomes protected β-carotene from degradation induced by radiation better than liposomes or dissolution in chloroform. With respect to the skin microstructure, no changes after irradiation were observed via freeze substitution transmission electron microscopy (FSTEM). This technique also showed the presence of vesicular structures in the stratum corneum (SC) after treatment with bicosomes.
基于磷脂的纳米结构，即双分子层囊泡和双联体脂质体，被提议作为抗氧化剂β-胡萝卜素的载体。在由紫外线A、可见光和红外线A辐射（UVA-VIS-IRA）诱导的氧化环境中，评估了这些纳米结构及其类胡萝卜素负载物的稳定性。此外，还研究了这些纳米聚集体对未辐照和辐照皮肤微观结构的影响。使用动态光散射（DLS）和冷冻透射电子显微镜（Cryo-TEM）对脂质体系进行了表征，并通过硫代巴比妥酸（TBARS）测定法确定了体系的脂质过氧化作用。此外，利用拉曼光谱研究了β-胡萝卜素在这些脂质体系中在这种辐射下的稳定性。结果表明，双分子层囊泡的粒径不会因辐射而改变。然而，双联体脂质体的粒径在辐照后略有增加。TBARS测定法表明双分子层囊泡和双联体脂质体中不存在过氧化物，这表明在所使用的辐射下脂质分子得以保存。拉曼实验表明，与脂质体或溶解于氯仿相比，双联体脂质体能更好地保护β-胡萝卜素免受辐射诱导的降解。关于皮肤微观结构，通过冷冻置换透射电子显微镜（FSTEM）观察到辐照后没有变化。该技术还显示在用双联体脂质体处理后的角质层（SC）中存在囊泡结构。
