Wang Tao, Lin Zhen, Ni Ming, Thien Christine, Day Robert E, Gardiner Bruce, Rubenson Jonas, Kirk Thomas B, Smith David W, Wang Allan, Lloyd David G, Wang Yan, Zheng Qiujian, Zheng Ming H
Division of Orthopaedic Surgery, Department of Surgery, Guangdong General Hospital, Guangdong Academy of Medicine Science, Guangzhou, Guangdong, China.
Centre for Orthopaedic Translational Research, School of Surgery, University of Western Australia, Nedlands, Australia.
J Orthop Res. 2015 Dec;33(12):1888-96. doi: 10.1002/jor.22960. Epub 2015 Jul 17.
Physiotherapy is one of the effective treatments for tendinopathy, whereby symptoms are relieved by changing the biomechanical environment of the pathological tendon. However, the underlying mechanism remains unclear. In this study, we first established a model of progressive tendinopathy-like degeneration in the rabbit Achilles. Following ex vivo loading deprivation culture in a bioreactor system for 6 and 12 days, tendons exhibited progressive degenerative changes, abnormal collagen type III production, increased cell apoptosis, and weakened mechanical properties. When intervention was applied at day 7 for another 6 days by using cyclic tensile mechanical stimulation (6% strain, 0.25 Hz, 8 h/day) in a bioreactor, the pathological changes and mechanical properties were almost restored to levels seen in healthy tendon. Our results indicated that a proper biomechanical environment was able to rescue early-stage pathological changes by increased collagen type I production, decreased collagen degradation and cell apoptosis. The ex vivo model developed in this study allows systematic study on the effect of mechanical stimulation on tendon biology.
物理治疗是肌腱病的有效治疗方法之一，通过改变病变肌腱的生物力学环境来缓解症状。然而，其潜在机制仍不清楚。在本研究中，我们首先建立了兔跟腱渐进性肌腱病样退变模型。在生物反应器系统中进行体外负荷剥夺培养6天和12天后，肌腱出现渐进性退变、III型胶原产生异常、细胞凋亡增加以及力学性能减弱。当在第7天通过在生物反应器中使用循环拉伸机械刺激（6%应变，0.25Hz，8小时/天）再干预6天时，病理变化和力学性能几乎恢复到健康肌腱的水平。我们的结果表明，适当的生物力学环境能够通过增加I型胶原产生、减少胶原降解和细胞凋亡来挽救早期病理变化。本研究中建立的体外模型允许对机械刺激对肌腱生物学的影响进行系统研究。
