Gu Zhao Wei, Wang Yun Xiu, Cao Zhi Wei
From the Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Shenyang City, Liaoning Province, China (ZWG, ZWC) and Hematological Laboratory, Shengjing Hospital, China Medical University, Shenyang City, Liaoning Province, China (YXW).
Medicine (Baltimore). 2015 Jul;94(26):e1050. doi: 10.1097/MD.0000000000001050.
The etiology and pathogenesis of respiratory epithelial adenomatoid hamartoma (REAH) remain poorly understood, although some reports have suggested that REAH features an inflammatory process. T-helper type 9 (Th9) cells are a newly identified subset of CD4 T-helper cells characterized by the expression of high levels of interleukin (IL)-9, which may promote inflammation. As REAH may involve an inflammatory process, we evaluated whether IL-9 and/or Th9 cells were present in REAH and compared the levels thereof to those of normal nasal mucosa. Eleven patients with REAH and 5 exhibiting cerebrospinal fluid leakage were included in the study. Flow cytometry was used to measure Th9 cell numbers, a cytometric bead assay was applied to measure IL-9 levels, and real-time polymerase chain reaction was used to quantify the levels of mRNA encoding IL-9. Th9 cells, IL-9 mRNA, and IL-9 were detected in all REAH and control samples. The proportion of Th9 cells in the patients with REAH was significantly greater than that in the controls. The expression levels of IL-9-encoding mRNA and IL-9 protein were significantly higher in the patients with REAH than in the controls. The Th9 cell subset was expanded, the synthesis of IL-9-encoding mRNA was upregulated, and IL-9 secretion was increased in REAH tissue, suggesting that Th9 cells play a central role in the pathogenesis of the disease.
尽管一些报告表明呼吸道上皮腺样错构瘤（REAH）具有炎症过程，但其病因和发病机制仍知之甚少。9型辅助性T细胞（Th9）是新发现的CD4辅助性T细胞亚群，其特征是高水平表达白细胞介素（IL）-9，这可能会促进炎症。由于REAH可能涉及炎症过程，我们评估了REAH中是否存在IL-9和/或Th9细胞，并将其水平与正常鼻黏膜的水平进行比较。该研究纳入了11例REAH患者和5例脑脊液漏患者。采用流式细胞术检测Th9细胞数量，应用细胞计数珠分析法检测IL-9水平，并使用实时聚合酶链反应定量编码IL-9的mRNA水平。在所有REAH和对照样本中均检测到Th9细胞、IL-9 mRNA和IL-9。REAH患者中Th9细胞的比例显著高于对照组。REAH患者中编码IL-9的mRNA和IL-9蛋白的表达水平显著高于对照组。REAH组织中Th9细胞亚群扩大，编码IL-9的mRNA合成上调，IL-9分泌增加，提示Th9细胞在该疾病的发病机制中起核心作用。
