Garo Lucien P, Beynon Vanessa, Murugaiyan Gopal
Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2017;1585:127-140. doi: 10.1007/978-1-4939-6877-0_10.
IL-9-producing Th9 cells are a novel subset of T helper cells that develop independently of other T helper subsets. Th9 cells have been implicated in the pathogenesis of allergic asthma and autoimmunity, while also serving as critical effector T cells in mediating antitumor immune responses. Concomitant presence of TGF-β and IL-4 lead to the differentiation of naïve CD4 T cells towards the Th9 phenotype. In addition, several cytokines, including IL-1β, IL-2, IL-25, and IL-33, further amplify Th9 responses. Negative regulators of Th9 cells include other cytokines such as IFN-γ, IL-23, and IL-27. Here, we describe a detailed protocol for the analysis of STAT molecules involved in the differentiation of Th9 cells and Th9 inhibition by IL-27.
产生白细胞介素-9（IL-9）的Th9细胞是一类新型辅助性T细胞亚群，其发育独立于其他辅助性T细胞亚群。Th9细胞与过敏性哮喘和自身免疫的发病机制有关，同时在介导抗肿瘤免疫反应中也是关键的效应T细胞。转化生长因子-β（TGF-β）和白细胞介素-4（IL-4）共同存在会使初始CD4 T细胞向Th9表型分化。此外，包括白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）、白细胞介素-25（IL-25）和白细胞介素-33（IL-33）在内的多种细胞因子会进一步增强Th9反应。Th9细胞的负调节因子包括其他细胞因子，如干扰素-γ（IFN-γ）、白细胞介素-23（IL-23）和白细胞介素-27（IL-27）。在此，我们描述了一个详细方案，用于分析参与Th9细胞分化及白细胞介素-27对Th9细胞抑制作用的信号转导和转录激活因子（STAT）分子。
