Th9细胞中信号转导和转录激活因子（STAT）分子的流式细胞术评估

Flow Cytometric Assessment of STAT Molecules in Th9 Cells.

作者信息

Garo Lucien P, Beynon Vanessa, Murugaiyan Gopal

机构信息

Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

出版信息

Methods Mol Biol. 2017;1585:127-140. doi: 10.1007/978-1-4939-6877-0_10.

DOI:10.1007/978-1-4939-6877-0_10

PMID:28477192

Abstract

IL-9-producing Th9 cells are a novel subset of T helper cells that develop independently of other T helper subsets. Th9 cells have been implicated in the pathogenesis of allergic asthma and autoimmunity, while also serving as critical effector T cells in mediating antitumor immune responses. Concomitant presence of TGF-β and IL-4 lead to the differentiation of naïve CD4 T cells towards the Th9 phenotype. In addition, several cytokines, including IL-1β, IL-2, IL-25, and IL-33, further amplify Th9 responses. Negative regulators of Th9 cells include other cytokines such as IFN-γ, IL-23, and IL-27. Here, we describe a detailed protocol for the analysis of STAT molecules involved in the differentiation of Th9 cells and Th9 inhibition by IL-27.

摘要

产生白细胞介素-9（IL-9）的Th9细胞是一类新型辅助性T细胞亚群，其发育独立于其他辅助性T细胞亚群。Th9细胞与过敏性哮喘和自身免疫的发病机制有关，同时在介导抗肿瘤免疫反应中也是关键的效应T细胞。转化生长因子-β（TGF-β）和白细胞介素-4（IL-4）共同存在会使初始CD4 T细胞向Th9表型分化。此外，包括白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）、白细胞介素-25（IL-25）和白细胞介素-33（IL-33）在内的多种细胞因子会进一步增强Th9反应。Th9细胞的负调节因子包括其他细胞因子，如干扰素-γ（IFN-γ）、白细胞介素-23（IL-23）和白细胞介素-27（IL-27）。在此，我们描述了一个详细方案，用于分析参与Th9细胞分化及白细胞介素-27对Th9细胞抑制作用的信号转导和转录激活因子（STAT）分子。