基于线粒体COI基因序列揭示，本土范围内的桃小食心虫（鳞翅目：蛀果蛾科）由两个同域隐存谱系组成。
Carposina sasakii (Lepidoptera: Carposinidae) in its Native Range Consists of Two Sympatric Cryptic Lineages as Revealed by Mitochondrial COI Gene Sequences.
作者信息
Wang J, Yu Y, Li L-L, Guo D, Tao Y-L, Chu D
机构信息
Key Laboratory for Plant Virology of Shandong Province, Plant Protection Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, P. R. China.
Key Laboratory for Plant Virology of Shandong Province, Plant Protection Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, P. R. China
出版信息
J Insect Sci. 2015 Jul 1;15(1). doi: 10.1093/jisesa/iev063. Print 2015.
The genetic differentiation and genetic structure of the peach fruit moth, Carposina sasakii Matsumura (Lepidoptera: Carposinidae), was investigated in China, where the moth is native. The mitochondrial cytochrome c oxidase I (COI) gene of 180 individuals from 16 collections were sequenced and analyzed. The results showed that two sympatric and cryptic mtDNA lineages existed within C. sasakii in China. The genetic differentiation has significant correlation with the geographical distance, but has no evidence for host plant associations. Our results of haplotype distribution suggest that the C. sasakii individuals can naturally move between areas, while the movement of individuals between long-distance locations may be associated with human activities such as the transport of fruit. Finally, an mitochondrial COI gene PCR-RFLP method was developed to differentiate the two cryptic mtDNA lineages within C. sasakii, which provides rapid and reliable tool for the future research of the two lineages.
在中国本土对桃小食心虫（Carposina sasakii Matsumura，鳞翅目：蛀果蛾科）的遗传分化和遗传结构进行了研究。对来自16个采集地的180个个体的线粒体细胞色素c氧化酶I（COI）基因进行了测序和分析。结果表明，中国的桃小食心虫存在两个同域且隐秘的线粒体DNA谱系。遗传分化与地理距离显著相关，但没有证据表明与寄主植物有关联。我们的单倍型分布结果表明，桃小食心虫个体能够在不同区域之间自然移动，而个体在远距离地点之间的移动可能与水果运输等人类活动有关。最后，开发了一种线粒体COI基因PCR-RFLP方法来区分桃小食心虫体内的两个隐秘线粒体DNA谱系，这为这两个谱系未来的研究提供了快速可靠的工具。
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