Ni Zhaohui, Xiang Fengyang, Huang Honglan, Wang Guoqing, Li Fan
Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medical Science, Jilin University, Changchun, Jilin 130021, P.R. China.
Biomed Rep. 2015 May;3(3):375-378. doi: 10.3892/br.2015.441. Epub 2015 Mar 5.
Measles and rubella virus are usually considered as the causative agents in patients with febrile rash illness (FRI). However, investigators have identified that enteroviruses are also associated with FRI, and the present study was undertaken to investigate this association. In the study, 20 throat swab samples were collected from patients suffering from rash and fever between April and July in 2013. The 20 samples tested negative for measles virus, but all were positive when reverse transcription-polymerase chain reaction was performed using enterovirus universal primers. Specifically, five tested positive for Coxsackie B3 virus (). The titers of the five CVB3 isolates were 10, 10, 10, 10 and 10 tissue culture infectious dose/100 µl respectively. The partial sequences of the five isolates were identical to each other and were closely associated with the CVB3/MKP and CVB3/Macocy strains, which are known to induce myocarditis and neonatal diseases of the central nervous system. In conclusion, CVB3 may cause symptoms of fever and rash and should be differentiated from measles, rubella and other infectious pathogens. The five CVB3 isolates described in the study were genetically similar to each other and to other local CVB3 strains. The results provide further data on the viral pathogen spectrum associated with FRI.
麻疹病毒和风疹病毒通常被认为是发热出疹性疾病（FRI）患者的病原体。然而，研究人员已经确定肠道病毒也与FRI有关，本研究旨在调查这种关联。在该研究中，于2013年4月至7月期间从患有皮疹和发热的患者中收集了20份咽拭子样本。这20份样本麻疹病毒检测呈阴性，但当使用肠道病毒通用引物进行逆转录-聚合酶链反应时，所有样本均呈阳性。具体而言，5份样本柯萨奇B3病毒检测呈阳性（）。这5株柯萨奇B3病毒分离株的滴度分别为10、10、10、10和10组织培养感染剂量/100微升。这5株分离株的部分序列彼此相同，并且与已知可诱发心肌炎和中枢神经系统新生儿疾病的柯萨奇B3/MKP和柯萨奇B3/Macocy菌株密切相关。总之，柯萨奇B3病毒可能引起发热和皮疹症状，应与麻疹、风疹和其他感染性病原体相鉴别。该研究中描述的5株柯萨奇B3病毒分离株彼此之间以及与其他本地柯萨奇B3病毒株在基因上相似。这些结果为与FRI相关的病毒病原体谱提供了进一步的数据。
